Transganglionic regulation of central terminals of dorsal root ganglion cells by nerve growth factor (NGF)

Bert Csillik, Martin E. Schwab, Hans Thoenen

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Blockade of axonal transport or transection of the rat sciatic nerve results in transganglionic degenerative atrophy (TDA) of nerve terminals containing fluoride-resistant acid phosphatase (FRAP) in the Rolando substance of the spinal cord. Application of vinblastine (9 μg) in a cuff around the sciatic nerve of adult rats blocked the retrograde transport of [125I]NGF in sensory fibers; this amount of vinblastine is identical to the threshold amount that induces TDA. Conversely, application of NGF to the proximal stump of the transected sciatic nerve prevented or delayed the occurrence of TDA as reflected by the maintenance of FRAP in the upper dorsal horn, that otherwise would inevitably disappear following the peripheral nerve lesion. These results suggest that endogenous NGF transported retrogradely in peripheral sensory fibers of the adult rat under normal conditions may be responsible for the regulation of the structural and functional integrity of the central terminals of these FRAP-containing primary sensory neurons and that TDA may be the consequence of the failure of NGF to reach the perikarya of these neurons.

Original languageEnglish
Pages (from-to)11-15
Number of pages5
JournalBrain Research
Volume331
Issue number1
DOIs
Publication statusPublished - Apr 1 1985

Fingerprint

Spinal Ganglia
Nerve Growth Factor
Atrophy
Sciatic Nerve
Acid Phosphatase
Fluorides
Vinblastine
Axonal Transport
Sensory Receptor Cells
Peripheral Nerves
Spinal Cord
Maintenance
Neurons

Keywords

  • axoplasmic transport
  • degeneration
  • dorsal root ganglion
  • nerve growth factor
  • spinal cord

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Transganglionic regulation of central terminals of dorsal root ganglion cells by nerve growth factor (NGF). / Csillik, Bert; Schwab, Martin E.; Thoenen, Hans.

In: Brain Research, Vol. 331, No. 1, 01.04.1985, p. 11-15.

Research output: Contribution to journalArticle

@article{8f9bbe8ddc50449eb286c2847fad8d70,
title = "Transganglionic regulation of central terminals of dorsal root ganglion cells by nerve growth factor (NGF)",
abstract = "Blockade of axonal transport or transection of the rat sciatic nerve results in transganglionic degenerative atrophy (TDA) of nerve terminals containing fluoride-resistant acid phosphatase (FRAP) in the Rolando substance of the spinal cord. Application of vinblastine (9 μg) in a cuff around the sciatic nerve of adult rats blocked the retrograde transport of [125I]NGF in sensory fibers; this amount of vinblastine is identical to the threshold amount that induces TDA. Conversely, application of NGF to the proximal stump of the transected sciatic nerve prevented or delayed the occurrence of TDA as reflected by the maintenance of FRAP in the upper dorsal horn, that otherwise would inevitably disappear following the peripheral nerve lesion. These results suggest that endogenous NGF transported retrogradely in peripheral sensory fibers of the adult rat under normal conditions may be responsible for the regulation of the structural and functional integrity of the central terminals of these FRAP-containing primary sensory neurons and that TDA may be the consequence of the failure of NGF to reach the perikarya of these neurons.",
keywords = "axoplasmic transport, degeneration, dorsal root ganglion, nerve growth factor, spinal cord",
author = "Bert Csillik and Schwab, {Martin E.} and Hans Thoenen",
year = "1985",
month = "4",
day = "1",
doi = "10.1016/0006-8993(85)90709-7",
language = "English",
volume = "331",
pages = "11--15",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Transganglionic regulation of central terminals of dorsal root ganglion cells by nerve growth factor (NGF)

AU - Csillik, Bert

AU - Schwab, Martin E.

AU - Thoenen, Hans

PY - 1985/4/1

Y1 - 1985/4/1

N2 - Blockade of axonal transport or transection of the rat sciatic nerve results in transganglionic degenerative atrophy (TDA) of nerve terminals containing fluoride-resistant acid phosphatase (FRAP) in the Rolando substance of the spinal cord. Application of vinblastine (9 μg) in a cuff around the sciatic nerve of adult rats blocked the retrograde transport of [125I]NGF in sensory fibers; this amount of vinblastine is identical to the threshold amount that induces TDA. Conversely, application of NGF to the proximal stump of the transected sciatic nerve prevented or delayed the occurrence of TDA as reflected by the maintenance of FRAP in the upper dorsal horn, that otherwise would inevitably disappear following the peripheral nerve lesion. These results suggest that endogenous NGF transported retrogradely in peripheral sensory fibers of the adult rat under normal conditions may be responsible for the regulation of the structural and functional integrity of the central terminals of these FRAP-containing primary sensory neurons and that TDA may be the consequence of the failure of NGF to reach the perikarya of these neurons.

AB - Blockade of axonal transport or transection of the rat sciatic nerve results in transganglionic degenerative atrophy (TDA) of nerve terminals containing fluoride-resistant acid phosphatase (FRAP) in the Rolando substance of the spinal cord. Application of vinblastine (9 μg) in a cuff around the sciatic nerve of adult rats blocked the retrograde transport of [125I]NGF in sensory fibers; this amount of vinblastine is identical to the threshold amount that induces TDA. Conversely, application of NGF to the proximal stump of the transected sciatic nerve prevented or delayed the occurrence of TDA as reflected by the maintenance of FRAP in the upper dorsal horn, that otherwise would inevitably disappear following the peripheral nerve lesion. These results suggest that endogenous NGF transported retrogradely in peripheral sensory fibers of the adult rat under normal conditions may be responsible for the regulation of the structural and functional integrity of the central terminals of these FRAP-containing primary sensory neurons and that TDA may be the consequence of the failure of NGF to reach the perikarya of these neurons.

KW - axoplasmic transport

KW - degeneration

KW - dorsal root ganglion

KW - nerve growth factor

KW - spinal cord

UR - http://www.scopus.com/inward/record.url?scp=0021946352&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021946352&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(85)90709-7

DO - 10.1016/0006-8993(85)90709-7

M3 - Article

C2 - 2580595

AN - SCOPUS:0021946352

VL - 331

SP - 11

EP - 15

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -