Transforming growth factor-β induced protein, βIG-H3, is present in degraded form and altered localization in lattice corneal dystrophy type I

Lili Takács, Péter Boross, J. Tőzsér, L. Módis, G. Tóth, A. Berta

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Abstract

Lattice corneal dystrophy type I (LCDI) is an inherited autosomal dominant local amyloidosis, restricted to the corneal stroma. Comparison of electrophoretic profiles of normal and dystrophic corneas revealed a 42 kD protein, which was present only in dystrophic corneas. The N-terminal sequence of this protein showed identity to transforming growth factor-β induced gene product (βIG-H3). A polyclonal antiserum was raised in chicken against a synthetic peptide identical to the N- terminal portion of βIG-H3. On immunoblots, the antiserum stained the 42 kD band, and also a 68 kD band corresponding to the reported molecular weight of the intact βIG-H3. In normal corneas, only the 68 kD band was present. Immunohistologically, the antiserum stained corneal subepithelial regions, including subepithelial deposits, in dystrophic corneas. In normal corneas, the staining was observed only in the epithelium. These results may reflect the role of βIG-H3 in extracellular matrix construction and/or amyloid formation.

Original languageEnglish
Pages (from-to)739-745
Number of pages7
JournalExperimental Eye Research
Volume66
Issue number6
DOIs
Publication statusPublished - Jun 1998

Fingerprint

Transforming Growth Factors
Cornea
Immune Sera
Proteins
Corneal Stroma
Amyloidosis
Amyloid
Extracellular Matrix
Chickens
Epithelium
Molecular Weight
Lattice corneal dystrophy type 1
Staining and Labeling
Peptides
Genes

Keywords

  • βIG-H3
  • Biochemistry
  • Immunohistology
  • Lattice corneal dystrophy type I
  • SDS- PAGE

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

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title = "Transforming growth factor-β induced protein, βIG-H3, is present in degraded form and altered localization in lattice corneal dystrophy type I",
abstract = "Lattice corneal dystrophy type I (LCDI) is an inherited autosomal dominant local amyloidosis, restricted to the corneal stroma. Comparison of electrophoretic profiles of normal and dystrophic corneas revealed a 42 kD protein, which was present only in dystrophic corneas. The N-terminal sequence of this protein showed identity to transforming growth factor-β induced gene product (βIG-H3). A polyclonal antiserum was raised in chicken against a synthetic peptide identical to the N- terminal portion of βIG-H3. On immunoblots, the antiserum stained the 42 kD band, and also a 68 kD band corresponding to the reported molecular weight of the intact βIG-H3. In normal corneas, only the 68 kD band was present. Immunohistologically, the antiserum stained corneal subepithelial regions, including subepithelial deposits, in dystrophic corneas. In normal corneas, the staining was observed only in the epithelium. These results may reflect the role of βIG-H3 in extracellular matrix construction and/or amyloid formation.",
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AU - Boross, Péter

AU - Tőzsér, J.

AU - Módis, L.

AU - Tóth, G.

AU - Berta, A.

PY - 1998/6

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N2 - Lattice corneal dystrophy type I (LCDI) is an inherited autosomal dominant local amyloidosis, restricted to the corneal stroma. Comparison of electrophoretic profiles of normal and dystrophic corneas revealed a 42 kD protein, which was present only in dystrophic corneas. The N-terminal sequence of this protein showed identity to transforming growth factor-β induced gene product (βIG-H3). A polyclonal antiserum was raised in chicken against a synthetic peptide identical to the N- terminal portion of βIG-H3. On immunoblots, the antiserum stained the 42 kD band, and also a 68 kD band corresponding to the reported molecular weight of the intact βIG-H3. In normal corneas, only the 68 kD band was present. Immunohistologically, the antiserum stained corneal subepithelial regions, including subepithelial deposits, in dystrophic corneas. In normal corneas, the staining was observed only in the epithelium. These results may reflect the role of βIG-H3 in extracellular matrix construction and/or amyloid formation.

AB - Lattice corneal dystrophy type I (LCDI) is an inherited autosomal dominant local amyloidosis, restricted to the corneal stroma. Comparison of electrophoretic profiles of normal and dystrophic corneas revealed a 42 kD protein, which was present only in dystrophic corneas. The N-terminal sequence of this protein showed identity to transforming growth factor-β induced gene product (βIG-H3). A polyclonal antiserum was raised in chicken against a synthetic peptide identical to the N- terminal portion of βIG-H3. On immunoblots, the antiserum stained the 42 kD band, and also a 68 kD band corresponding to the reported molecular weight of the intact βIG-H3. In normal corneas, only the 68 kD band was present. Immunohistologically, the antiserum stained corneal subepithelial regions, including subepithelial deposits, in dystrophic corneas. In normal corneas, the staining was observed only in the epithelium. These results may reflect the role of βIG-H3 in extracellular matrix construction and/or amyloid formation.

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