Transformation vector based on promoter and intron sequences of a replacement histone H3 gene. A tool for high, constitutive gene expression in plants

Zsolt Kelemen, Antal Mai, Tamás Kapros, A. Fehér, J. Györgyey, Jakob H. Waterborg, D. Dudits

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This study explored the possibility of using non-viral, plant-based gene sequences to create strong and constitutive expression vectors. Replacement histone H3 genes are highly and constitutively expressed in all plants. Sequences of the cloned alfalfa histone H3.2 gene MsH3g1 were tested. Constructs of the β-glucuronidase (GUS) reporter gene were produced with H3.2 gene promoter and intron sequences. Their efficiency was compared with that of the commonly used strong 35S cauliflower mosaic virus promoter in transgenic tobacco plants. Combination of the H3.2 promoter and intron produced significantly higher GUS expression than the strong viral 35S promoter. Histochemical GUS analysis revealed a constitutive pattern of expression. Thus, alfalfa replacement H3 gene sequences can be used instead of viral promoters to drive heterologous gene expression in plants, avoiding perceived risks of viral sequences.

Original languageEnglish
Pages (from-to)69-72
Number of pages4
JournalTransgenic Research
Volume11
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

histones
Histones
Introns
introns
Glucuronidase
promoter regions
Gene Expression
gene expression
Medicago sativa
Genes
genes
Caulimovirus
alfalfa
Plant Genes
Genetically Modified Plants
nucleotide sequences
Reporter Genes
risk perception
Cauliflower mosaic virus
Tobacco

Keywords

  • Alfalfa
  • GUS reporter
  • Histone H3
  • Intron
  • Promoter strength
  • Transgene

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Biotechnology
  • Food Science

Cite this

Transformation vector based on promoter and intron sequences of a replacement histone H3 gene. A tool for high, constitutive gene expression in plants. / Kelemen, Zsolt; Mai, Antal; Kapros, Tamás; Fehér, A.; Györgyey, J.; Waterborg, Jakob H.; Dudits, D.

In: Transgenic Research, Vol. 11, No. 1, 2002, p. 69-72.

Research output: Contribution to journalArticle

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