Transformation using controlled cDNA overexpression system.

Gábor Rigó, Csaba Papdi, László Szabados

Research output: Contribution to journalArticle

Abstract

The controlled cDNA overexpression system (COS) was developed to identify novel regulatory genes in model plants as well as in other species that might have a particular valuable trait. The COS system (Papdi et al. Plant Physiol 147:528-542, 2008) is composed of a random cDNA library prepared in a T-DNA plant expression vector, under the control of the estradiol-inducible XVE promoter. Large-scale genetic transformation of Arabidopsis thaliana generates a transgenic plant population with randomly inserted cDNA clones. Overexpression of the inserted cDNA can create selectable phenotypes, allowing the facile identification and cloning of the responsible genes. Here we describe protocols to create and use the COS system for diverse purposes in plant biology.

Original languageEnglish
Pages (from-to)277-290
Number of pages14
JournalMethods in molecular biology (Clifton, N.J.)
Volume913
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Complementary DNA
Genetic Transformation
Genetically Modified Plants
Regulator Genes
Gene Library
Arabidopsis
Organism Cloning
Estradiol
Clone Cells
Phenotype
Population
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Transformation using controlled cDNA overexpression system. / Rigó, Gábor; Papdi, Csaba; Szabados, László.

In: Methods in molecular biology (Clifton, N.J.), Vol. 913, 2012, p. 277-290.

Research output: Contribution to journalArticle

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