Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress

Patcharaporn Deshnium, Dmitry A. Los, Hidenori Hayashi, L. Mustárdy, Norio Murata

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Choline oxidase, isolated from the soil bacterium Arthrobacter globiformis, converts choline to glycinebetaine (N-trimethylglycine) without a requirement for any cofactors. The gene for this enzyme, designated codA, was cloned and introduced into the cyanobacterium Synechococcus sp. PCC 7942. The codA gene was experssed under the control of a strong constitutive promoter, and the transformed cells accumulated glycinebetaine at intracellular levels of 60-80 mM. Consequently the cells acquired tolerance to salt stress, as evaluated in terms of growth, accumulation of chlorophyll and photosynthetic activity.

Original languageEnglish
Pages (from-to)897-907
Number of pages11
JournalPlant Molecular Biology
Volume29
Issue number5
DOIs
Publication statusPublished - Dec 1995

Fingerprint

choline oxidase
Synechococcus
betaine
salt stress
Arthrobacter globiformis
Salts
Genes
Synechococcus sp. PCC 7942
Arthrobacter
soil bacteria
Cyanobacteria
Chlorophyll
choline
Choline
Bacteria
Soil
genes
promoter regions
cells
Soils

Keywords

  • Arthrobacter globiformis
  • choline oxidase
  • codA gene
  • glycinebetaine
  • salt tolerance
  • Synechococcus

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress. / Deshnium, Patcharaporn; Los, Dmitry A.; Hayashi, Hidenori; Mustárdy, L.; Murata, Norio.

In: Plant Molecular Biology, Vol. 29, No. 5, 12.1995, p. 897-907.

Research output: Contribution to journalArticle

Deshnium, Patcharaporn ; Los, Dmitry A. ; Hayashi, Hidenori ; Mustárdy, L. ; Murata, Norio. / Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress. In: Plant Molecular Biology. 1995 ; Vol. 29, No. 5. pp. 897-907.
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