Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress

Patcharaporn Deshnium, Dmitry A. Los, Hidenori Hayashi, Laszlo Mustardy, Norio Murata

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Abstract

Choline oxidase, isolated from the soil bacterium Arthrobacter globiformis, converts choline to glycinebetaine (N-trimethylglycine) without a requirement for any cofactors. The gene for this enzyme, designated codA, was cloned and introduced into the cyanobacterium Synechococcus sp. PCC 7942. The codA gene was experssed under the control of a strong constitutive promoter, and the transformed cells accumulated glycinebetaine at intracellular levels of 60-80 mM. Consequently the cells acquired tolerance to salt stress, as evaluated in terms of growth, accumulation of chlorophyll and photosynthetic activity.

Original languageEnglish
Pages (from-to)897-907
Number of pages11
JournalPlant molecular biology
Volume29
Issue number5
DOIs
Publication statusPublished - Dec 1 1995

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Keywords

  • Arthrobacter globiformis
  • Synechococcus
  • choline oxidase
  • codA gene
  • glycinebetaine
  • salt tolerance

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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