Transformation of floral organs with GFP in Medicago truncatula

K. Kamaté, I. D. Rodriguez-Llorente, M. Scholte, P. Durand, P. Ratet, E. Kondorosi, A. Kondorosi, T. H. Trinh

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39 Citations (Scopus)

Abstract

A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this method allows us to save time and to use a smaller greenhouse surface for the culture of donor plants. Southern hybridization showed that the internal gfp fragment had the expected size and the number of T-DNA copies integrated in the plant genome varied between one and three. These data suggest that the presence of the GFP protein has no toxic effects, since no rearrangement of the gfp reporter gene was detected in the regenerated plants.

Original languageEnglish
Pages (from-to)647-653
Number of pages7
JournalPlant Cell Reports
Volume19
Issue number7
DOIs
Publication statusPublished - Jun 1 2000

Keywords

  • Floral organs
  • Medicago truncatula
  • gfp

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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  • Cite this

    Kamaté, K., Rodriguez-Llorente, I. D., Scholte, M., Durand, P., Ratet, P., Kondorosi, E., Kondorosi, A., & Trinh, T. H. (2000). Transformation of floral organs with GFP in Medicago truncatula. Plant Cell Reports, 19(7), 647-653. https://doi.org/10.1007/s002999900168