Transformation of floral organs with GFP in Medicago truncatula

K. Kamaté, I. D. Rodriguez-Llorente, M. Scholte, P. Durand, P. Ratet, É. Kondorosi, A. Kondorosi, T. H. Trinh

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this method allows us to save time and to use a smaller greenhouse surface for the culture of donor plants. Southern hybridization showed that the internal gfp fragment had the expected size and the number of T-DNA copies integrated in the plant genome varied between one and three. These data suggest that the presence of the GFP protein has no toxic effects, since no rearrangement of the gfp reporter gene was detected in the regenerated plants.

Original languageEnglish
Pages (from-to)647-653
Number of pages7
JournalPlant Cell Reports
Volume19
Issue number7
DOIs
Publication statusPublished - Jun 2000

Fingerprint

Medicago truncatula
reporter genes
flowers
gene fusion
Southern blotting
transgenic plants
embryogenesis
promoter regions
greenhouses
genome
color
DNA
proteins
cells
methodology

Keywords

  • Floral organs
  • gfp
  • Medicago truncatula

ASJC Scopus subject areas

  • Plant Science

Cite this

Kamaté, K., Rodriguez-Llorente, I. D., Scholte, M., Durand, P., Ratet, P., Kondorosi, É., ... Trinh, T. H. (2000). Transformation of floral organs with GFP in Medicago truncatula. Plant Cell Reports, 19(7), 647-653. https://doi.org/10.1007/s002999900168

Transformation of floral organs with GFP in Medicago truncatula. / Kamaté, K.; Rodriguez-Llorente, I. D.; Scholte, M.; Durand, P.; Ratet, P.; Kondorosi, É.; Kondorosi, A.; Trinh, T. H.

In: Plant Cell Reports, Vol. 19, No. 7, 06.2000, p. 647-653.

Research output: Contribution to journalArticle

Kamaté, K, Rodriguez-Llorente, ID, Scholte, M, Durand, P, Ratet, P, Kondorosi, É, Kondorosi, A & Trinh, TH 2000, 'Transformation of floral organs with GFP in Medicago truncatula', Plant Cell Reports, vol. 19, no. 7, pp. 647-653. https://doi.org/10.1007/s002999900168
Kamaté K, Rodriguez-Llorente ID, Scholte M, Durand P, Ratet P, Kondorosi É et al. Transformation of floral organs with GFP in Medicago truncatula. Plant Cell Reports. 2000 Jun;19(7):647-653. https://doi.org/10.1007/s002999900168
Kamaté, K. ; Rodriguez-Llorente, I. D. ; Scholte, M. ; Durand, P. ; Ratet, P. ; Kondorosi, É. ; Kondorosi, A. ; Trinh, T. H. / Transformation of floral organs with GFP in Medicago truncatula. In: Plant Cell Reports. 2000 ; Vol. 19, No. 7. pp. 647-653.
@article{d73dc8ea3ac7434181dce98f05092af6,
title = "Transformation of floral organs with GFP in Medicago truncatula",
abstract = "A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this method allows us to save time and to use a smaller greenhouse surface for the culture of donor plants. Southern hybridization showed that the internal gfp fragment had the expected size and the number of T-DNA copies integrated in the plant genome varied between one and three. These data suggest that the presence of the GFP protein has no toxic effects, since no rearrangement of the gfp reporter gene was detected in the regenerated plants.",
keywords = "Floral organs, gfp, Medicago truncatula",
author = "K. Kamat{\'e} and Rodriguez-Llorente, {I. D.} and M. Scholte and P. Durand and P. Ratet and {\'E}. Kondorosi and A. Kondorosi and Trinh, {T. H.}",
year = "2000",
month = "6",
doi = "10.1007/s002999900168",
language = "English",
volume = "19",
pages = "647--653",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "7",

}

TY - JOUR

T1 - Transformation of floral organs with GFP in Medicago truncatula

AU - Kamaté, K.

AU - Rodriguez-Llorente, I. D.

AU - Scholte, M.

AU - Durand, P.

AU - Ratet, P.

AU - Kondorosi, É.

AU - Kondorosi, A.

AU - Trinh, T. H.

PY - 2000/6

Y1 - 2000/6

N2 - A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this method allows us to save time and to use a smaller greenhouse surface for the culture of donor plants. Southern hybridization showed that the internal gfp fragment had the expected size and the number of T-DNA copies integrated in the plant genome varied between one and three. These data suggest that the presence of the GFP protein has no toxic effects, since no rearrangement of the gfp reporter gene was detected in the regenerated plants.

AB - A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this method allows us to save time and to use a smaller greenhouse surface for the culture of donor plants. Southern hybridization showed that the internal gfp fragment had the expected size and the number of T-DNA copies integrated in the plant genome varied between one and three. These data suggest that the presence of the GFP protein has no toxic effects, since no rearrangement of the gfp reporter gene was detected in the regenerated plants.

KW - Floral organs

KW - gfp

KW - Medicago truncatula

UR - http://www.scopus.com/inward/record.url?scp=0033935728&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033935728&partnerID=8YFLogxK

U2 - 10.1007/s002999900168

DO - 10.1007/s002999900168

M3 - Article

AN - SCOPUS:0033935728

VL - 19

SP - 647

EP - 653

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 7

ER -