Transfer of isolated nuclei into protoplasts of Saccharomyces cerevisiae

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Abstract

Cell nuclei were prepared from protoplasts of an adenine-requiring strain of Saccharomyces cerevisiae, then purified in a discontinuous sucrose gradient, and applied to protoplasts of a recipient strain auxotrophic for uracil, leucine, and histidine. The transfer of the isolated nuclei into protoplasts was induced with polyethylene glycol. The main products of nuclear transfer in young complemented colonies were heterokaryons giving rise to parental type spontaneuos segregants on nutritionally complete medium. After several passages in minimal medium, however, the prototrophic colonies consisted exclusively of stable heterozygous diploid cells.

Original languageEnglish
Pages (from-to)157-160
Number of pages4
JournalCurrent Microbiology
Volume7
Issue number3
DOIs
Publication statusPublished - 1982

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Protoplasts
Saccharomyces cerevisiae
Uracil
Adenine
Cell Nucleus
Diploidy
Histidine
Leucine
Sucrose

ASJC Scopus subject areas

  • Microbiology

Cite this

Transfer of isolated nuclei into protoplasts of Saccharomyces cerevisiae. / Ferenczy, L.; Pesti, M.

In: Current Microbiology, Vol. 7, No. 3, 1982, p. 157-160.

Research output: Contribution to journalArticle

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