Transduction of Porcine Enteropathogenic Escherichia coli with a Derivative of a Shiga Toxin 2-Encoding Bacteriophage in a Porcine Ligated Ileal Loop System

I. Tóth, Herbert Schmidt, Mohamed Dow, Anna Malik, Eric Oswald, B. Nagy

Research output: Contribution to journalArticle

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Abstract

In this study, we have investigated the ability of detoxified Shiga toxin (Stx)-converting bacteriophages Φ3538 (Δstx2::cat) (H. Schmidt et al., A ppl. Environ. Microbiol. 65:3855-3861, 1999) and H-19B::Tn10d-bla (D. W. Acheson et al., Infect. Immun. 66:4496-4498, 1998) to lysogenize enteropathogenic Escherichia coli (EPEC) strains in vivo. We were able to transduce the porcine EPEC strain 1390 (045) with Φ3538 (Δstx2::cat) in porcine ligated ileal loops but not the human EPEC prototype strain E234/69 (O127). Neither strain 1390 nor strain E2348/69 was lysogenized under these in vivo conditions when E. coli K-12 containing H-19B::Tn10d.bla was used as the stx1 phage donor. The repeated success in the in vivo transduction of an Stx2-encoding phage to a porcine EPEC strain in pig loops was in contrast to failures in the in vitro trials with these and other EPEC strains. These results indicate that in vivo conditions are more effective for transduction of Stx2-encoding phages than in vitro conditions.

Original languageEnglish
Pages (from-to)7242-7247
Number of pages6
JournalApplied and Environmental Microbiology
Volume69
Issue number12
DOIs
Publication statusPublished - Dec 2003

Fingerprint

Shiga Toxin 2
Shiga-like toxin 2
Enteropathogenic Escherichia coli
enteropathogenic Escherichia coli
bacteriophage
pig
bacteriophages
Bacteriophages
toxin
Swine
swine
Cats
Shiga Toxin
cats
Escherichia coli K12
Shiga toxin
trial
prototypes
Escherichia coli

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Transduction of Porcine Enteropathogenic Escherichia coli with a Derivative of a Shiga Toxin 2-Encoding Bacteriophage in a Porcine Ligated Ileal Loop System. / Tóth, I.; Schmidt, Herbert; Dow, Mohamed; Malik, Anna; Oswald, Eric; Nagy, B.

In: Applied and Environmental Microbiology, Vol. 69, No. 12, 12.2003, p. 7242-7247.

Research output: Contribution to journalArticle

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