Transdifferentiation and Proliferation in Two Distinct Hemocyte Lineages in Drosophila melanogaster Larvae after Wasp Infection

Ines Anderl, Laura Vesala, Teemu O. Ihalainen, Leena Maija Vanha-aho, I. Andó, Mika Rämet, Dan Hultmark

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Cellular immune responses require the generation and recruitment of diverse blood cell types that recognize and kill pathogens. In Drosophila melanogaster larvae, immune-inducible lamellocytes participate in recognizing and killing parasitoid wasp eggs. However, the sequence of events required for lamellocyte generation remains controversial. To study the cellular immune system, we developed a flow cytometry approach using in vivo reporters for lamellocytes as well as for plasmatocytes, the main hemocyte type in healthy larvae. We found that two different blood cell lineages, the plasmatocyte and lamellocyte lineages, contribute to the generation of lamellocytes in a demand-adapted hematopoietic process. Plasmatocytes transdifferentiate into lamellocyte-like cells in situ directly on the wasp egg. In parallel, a novel population of infection-induced cells, which we named lamelloblasts, appears in the circulation. Lamelloblasts proliferate vigorously and develop into the major class of circulating lamellocytes. Our data indicate that lamellocyte differentiation upon wasp parasitism is a plastic and dynamic process. Flow cytometry with in vivo hemocyte reporters can be used to study this phenomenon in detail.

Original languageEnglish
Article numbere1005746
JournalPLoS Pathogens
Volume12
Issue number7
DOIs
Publication statusPublished - Jul 1 2016

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Hemocytes
Wasps
Drosophila melanogaster
Larva
Blood Cells
Flow Cytometry
Infection
Cell Lineage
Cellular Immunity
Eggs
Plastics
Ovum
Immune System
Population

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Transdifferentiation and Proliferation in Two Distinct Hemocyte Lineages in Drosophila melanogaster Larvae after Wasp Infection. / Anderl, Ines; Vesala, Laura; Ihalainen, Teemu O.; Vanha-aho, Leena Maija; Andó, I.; Rämet, Mika; Hultmark, Dan.

In: PLoS Pathogens, Vol. 12, No. 7, e1005746, 01.07.2016.

Research output: Contribution to journalArticle

Anderl, Ines ; Vesala, Laura ; Ihalainen, Teemu O. ; Vanha-aho, Leena Maija ; Andó, I. ; Rämet, Mika ; Hultmark, Dan. / Transdifferentiation and Proliferation in Two Distinct Hemocyte Lineages in Drosophila melanogaster Larvae after Wasp Infection. In: PLoS Pathogens. 2016 ; Vol. 12, No. 7.
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