Transcriptome analysis of potato leaves expressing the trehalose-6-phosphate synthase 1 gene of yeast

Mihály Kondrák, Ferenc Marincs, Balázs Kalapos, Zsófia Juhász, Z. Bánfalvi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Transgenic lines of the potato cultivar White Lady expressing the trehalose-6-phosphate synthase (TPS1) gene of yeast exhibit improved drought tolerance, but grow slower and have a lower carbon fixation rate and stomatal density than the wild-type. To understand the molecular basis of this phenomenon, we have compared the transcriptomes of wild-type and TPS1-transgenic plants using the POCI microarray containing 42,034 potato unigene probes. We show that 74 and 25 genes were up-, and down-regulated, respectively, in the mature source leaves of TPS1-transgenic plants when compared with the wild-type. The differentially regulated genes were assigned into 16 functional groups. All of the seven genes, which were assigned into carbon fixation and metabolism group, were up-regulated, while about 42% of the assigned genes are involved in transcriptional and post-transcriptional regulation. Expression of genes encoding a 14-3-3 regulatory protein, and four transcription factors were down-regulated in the TPS1-transgenic leaves. To verify the microarray results, we used RNA gel blot analysis to examine the expression of eight genes and found that the RNA gel blot and microarray data correlated in each case. Using the putative Arabidopsis orthologs of the assigned potato sequences we have identified putative transcription binding sites in the promoter region of the differentially regulated genes, and putative protein-protein interactions involving some of the up- and down-regulated genes. We have also demonstrated that starch content is lower, while malate, inositol and maltose contents are higher in the TPS1-transgenic than in the wild-type leaves. Our results suggest that a complex regulatory network, involving transcription factors and other regulatory proteins, underpins the phenotypic alterations we have observed previously in potato when expressing the TPS1 gene of yeast.

Original languageEnglish
Article numbere23466
JournalPLoS One
Volume6
Issue number8
DOIs
Publication statusPublished - 2011

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Gene Expression Profiling
Solanum tuberosum
transcriptomics
Yeast
Genes
Yeasts
potatoes
yeasts
leaves
genes
Carbon Cycle
Microarrays
Genetically Modified Plants
regulatory proteins
genetically modified organisms
Transcription Factors
Gels
transgenic plants
RNA
transcription factors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Transcriptome analysis of potato leaves expressing the trehalose-6-phosphate synthase 1 gene of yeast. / Kondrák, Mihály; Marincs, Ferenc; Kalapos, Balázs; Juhász, Zsófia; Bánfalvi, Z.

In: PLoS One, Vol. 6, No. 8, e23466, 2011.

Research output: Contribution to journalArticle

Kondrák, Mihály ; Marincs, Ferenc ; Kalapos, Balázs ; Juhász, Zsófia ; Bánfalvi, Z. / Transcriptome analysis of potato leaves expressing the trehalose-6-phosphate synthase 1 gene of yeast. In: PLoS One. 2011 ; Vol. 6, No. 8.
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