Transcriptome analysis of a bacterially induced basal and hypersensitive response of Medicago truncatula

Zoltán Bozsó, Nicolas Maunoury, Agnes Szatmari, Peter Mergaert, P. Ott, László R. Zsíros, Erika Szabó, É. Kondorosi, Zoltán Klement

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Research using the well-studied model legume Medicago truncatula has largely focused on rhizobium symbiosis, while little information is currently available for this species on pathogen-induced transcriptome changes. We have performed a transcriptome analysis of this species with the objective of studying the basal (BR, no visible symptoms) and hypersensitive response (HR, plant cell death) in its leaves at 6 and at 24 h after infection by HR-negative (hrcC mutant) and HR-inducing Pseudomonas syringae pv. syringae strains, respectively. Although there were no visible symptoms at the BR, the alterations in gene expression were comparable to those found with the HR. Both responses resulted in the transcriptional alteration of hundreds of plant genes; however, the responses in the HR were usually more intense. The reactions to HR-inducing and HR-negative bacterial strains were significantly overlapping. Parallel up- or down-regulation of genes with the same function occurred frequently. However, some plant processes were regulated in one direction; for example, most of the protein synthesis-related genes were activated and all of the photosynthetic/chloroplast genes were suppressed during BR. The possible roles of several functional classes (e.g., cell rescue, signaling, defense, cell death, etc.) of transcriptionally altered genes are discussed. The results of the comparison with available mycorrhizal and nodule expression data show that there is a significant overlap between nodulation and the leaf defense response and that during the early stage of the nodulation in roots, Sinorhizobium meliloti induces a fluctuation in the transcription of BR- and HR-responsive genes.

Original languageEnglish
Pages (from-to)627-646
Number of pages20
JournalPlant Molecular Biology
Volume70
Issue number6
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Medicago truncatula
Gene Expression Profiling
hypersensitive response
transcriptomics
Genes
Cell Death
genes
Chloroplast Genes
Sinorhizobium meliloti
Pseudomonas syringae
nodulation
Plant Genes
signs and symptoms (plants)
Rhizobium
Symbiosis
cell death
Plant Cells
Transcriptome
Fabaceae
Pseudomonas syringae pv. syringae

Keywords

  • Basal resistance
  • Medicago truncatula
  • Microarray
  • Mycorrhiza
  • Nodulation
  • Pseudomonas syringae

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Genetics

Cite this

Transcriptome analysis of a bacterially induced basal and hypersensitive response of Medicago truncatula. / Bozsó, Zoltán; Maunoury, Nicolas; Szatmari, Agnes; Mergaert, Peter; Ott, P.; Zsíros, László R.; Szabó, Erika; Kondorosi, É.; Klement, Zoltán.

In: Plant Molecular Biology, Vol. 70, No. 6, 08.2009, p. 627-646.

Research output: Contribution to journalArticle

Bozsó, Zoltán ; Maunoury, Nicolas ; Szatmari, Agnes ; Mergaert, Peter ; Ott, P. ; Zsíros, László R. ; Szabó, Erika ; Kondorosi, É. ; Klement, Zoltán. / Transcriptome analysis of a bacterially induced basal and hypersensitive response of Medicago truncatula. In: Plant Molecular Biology. 2009 ; Vol. 70, No. 6. pp. 627-646.
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