Transcriptome analysis indicates an enhanced activation of adaptive and innate immunity by Chlamydia-infected murine epithelial cells treated with interferon γ

K. Burián, V. Endrész, Judit Deak, Zsolt Kormanyos, A. Pál, David Nelson, D. Virók

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background. Interferon γ (IFN-γ) is the major cytokine involved in the elimination of Chlamydia infection. Despite its importance, the combined effect of Chlamydia infection and IFN-γ on the gene expression of murine epithelial cells has only partially been described. Methods. The DNA chip method was used to evaluate the impact of IFN-γ and both the human strain Chlamydia trachomatis L2 infection and the murine strain Chlamydia muridarum infection on the transcriptome of murine epithelial cells. Results. The gene expression analysis revealed that IFN-γ had an enhancing effect on both the up-regulation and down-regulation of the epithelial gene expression. The influenced gene functional classes included cytokine and chemokine expression, antigen presentation, apoptosis, and genes involved in basic metabolic processes such as fatty acid oxidation. We also detected the up-regulation of various genes that could be directly antichlamydial, such as members of the p47 GTPase family, inducible nitric oxide synthase, and monokine induced by IFN-γ (MIG). As a functional validation of DNA chip data, we measured the antichlamydial effect of MIG on the extracellular form of Chlamydia. Conclusions. Our results show that IFN-γ is a key cytokine that primes epithelial cells to activate adaptive and innate immunity and to express antichlamydial effector genes both intracellularly and extracellularly.

Original languageEnglish
Pages (from-to)1405-1414
Number of pages10
JournalJournal of Infectious Diseases
Volume202
Issue number9
DOIs
Publication statusPublished - Nov 1 2010

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Chlamydia
Gene Expression Profiling
Adaptive Immunity
Innate Immunity
Interferons
Chlamydia Infections
Epithelial Cells
Cytokines
Oligonucleotide Array Sequence Analysis
Genes
Chlamydia muridarum
Up-Regulation
Monokines
Gene Expression
GTP Phosphohydrolases
Chlamydia trachomatis
Antigen Presentation
Gene Expression Regulation
Nitric Oxide Synthase Type II
Transcriptome

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Medicine(all)

Cite this

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title = "Transcriptome analysis indicates an enhanced activation of adaptive and innate immunity by Chlamydia-infected murine epithelial cells treated with interferon γ",
abstract = "Background. Interferon γ (IFN-γ) is the major cytokine involved in the elimination of Chlamydia infection. Despite its importance, the combined effect of Chlamydia infection and IFN-γ on the gene expression of murine epithelial cells has only partially been described. Methods. The DNA chip method was used to evaluate the impact of IFN-γ and both the human strain Chlamydia trachomatis L2 infection and the murine strain Chlamydia muridarum infection on the transcriptome of murine epithelial cells. Results. The gene expression analysis revealed that IFN-γ had an enhancing effect on both the up-regulation and down-regulation of the epithelial gene expression. The influenced gene functional classes included cytokine and chemokine expression, antigen presentation, apoptosis, and genes involved in basic metabolic processes such as fatty acid oxidation. We also detected the up-regulation of various genes that could be directly antichlamydial, such as members of the p47 GTPase family, inducible nitric oxide synthase, and monokine induced by IFN-γ (MIG). As a functional validation of DNA chip data, we measured the antichlamydial effect of MIG on the extracellular form of Chlamydia. Conclusions. Our results show that IFN-γ is a key cytokine that primes epithelial cells to activate adaptive and innate immunity and to express antichlamydial effector genes both intracellularly and extracellularly.",
author = "K. Buri{\'a}n and V. Endr{\'e}sz and Judit Deak and Zsolt Kormanyos and A. P{\'a}l and David Nelson and D. Vir{\'o}k",
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T1 - Transcriptome analysis indicates an enhanced activation of adaptive and innate immunity by Chlamydia-infected murine epithelial cells treated with interferon γ

AU - Burián, K.

AU - Endrész, V.

AU - Deak, Judit

AU - Kormanyos, Zsolt

AU - Pál, A.

AU - Nelson, David

AU - Virók, D.

PY - 2010/11/1

Y1 - 2010/11/1

N2 - Background. Interferon γ (IFN-γ) is the major cytokine involved in the elimination of Chlamydia infection. Despite its importance, the combined effect of Chlamydia infection and IFN-γ on the gene expression of murine epithelial cells has only partially been described. Methods. The DNA chip method was used to evaluate the impact of IFN-γ and both the human strain Chlamydia trachomatis L2 infection and the murine strain Chlamydia muridarum infection on the transcriptome of murine epithelial cells. Results. The gene expression analysis revealed that IFN-γ had an enhancing effect on both the up-regulation and down-regulation of the epithelial gene expression. The influenced gene functional classes included cytokine and chemokine expression, antigen presentation, apoptosis, and genes involved in basic metabolic processes such as fatty acid oxidation. We also detected the up-regulation of various genes that could be directly antichlamydial, such as members of the p47 GTPase family, inducible nitric oxide synthase, and monokine induced by IFN-γ (MIG). As a functional validation of DNA chip data, we measured the antichlamydial effect of MIG on the extracellular form of Chlamydia. Conclusions. Our results show that IFN-γ is a key cytokine that primes epithelial cells to activate adaptive and innate immunity and to express antichlamydial effector genes both intracellularly and extracellularly.

AB - Background. Interferon γ (IFN-γ) is the major cytokine involved in the elimination of Chlamydia infection. Despite its importance, the combined effect of Chlamydia infection and IFN-γ on the gene expression of murine epithelial cells has only partially been described. Methods. The DNA chip method was used to evaluate the impact of IFN-γ and both the human strain Chlamydia trachomatis L2 infection and the murine strain Chlamydia muridarum infection on the transcriptome of murine epithelial cells. Results. The gene expression analysis revealed that IFN-γ had an enhancing effect on both the up-regulation and down-regulation of the epithelial gene expression. The influenced gene functional classes included cytokine and chemokine expression, antigen presentation, apoptosis, and genes involved in basic metabolic processes such as fatty acid oxidation. We also detected the up-regulation of various genes that could be directly antichlamydial, such as members of the p47 GTPase family, inducible nitric oxide synthase, and monokine induced by IFN-γ (MIG). As a functional validation of DNA chip data, we measured the antichlamydial effect of MIG on the extracellular form of Chlamydia. Conclusions. Our results show that IFN-γ is a key cytokine that primes epithelial cells to activate adaptive and innate immunity and to express antichlamydial effector genes both intracellularly and extracellularly.

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