Transcriptional repression of the human galactocerebrosidase gene in squamous cell carcinomas of the larynx

Tibor Görögh, Heinrich Rudert, Burkard M. Lippert, Stefan Gottschlich, Steffen Maune, Klaus Heidorn, Jan Maass, Markus Hoffmann, Jens E. Meyer, Immo O. Rathcke, Benedikt J. Folz, T. Hortobágyi, Jochen A. Werner

Research output: Contribution to journalArticle

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Abstract

Alterations of gene expression in squamous cell carcinoma (SCC) cell lines derived from the larynx and keratinocytes derived from adjacent normal mucosa of the larynx have been studied using the mRNA differential display technique. Lane-to-lane comparison of reverse transcribed mRNA showed a strong repression of a 148 bp fragment in SCC cells. The fragment was reamplified and cloned. Sequencing revealed a 99.3% homology with a region in exon 17 of the human galactocerebrosidase (GALC) gene. Northern blot analysis confirmed the differential expression of this gene in both carcinoma cell lines and laryngeal SCC biopsies in contrast with corresponding normal mucosa. To provide further evidence for the differential expression rate, both types of cells were transiently transfected with a 152 bp (-176 to -24) high regulatory promoter element of the 5' flanking region of the GALC gene. Results of 3 independent transfection experiments indicated a 16-fold repression of the GALC gene expression in SCC cells compared with benign keratinocytes. However, neither mutation nor other alterations of the promoter sequence were detected. Expression of the GALC gene is thus greatly affected in SCCs of the larynx.

Original languageEnglish
Pages (from-to)750-754
Number of pages5
JournalInternational Journal of Cancer
Volume83
Issue number6
DOIs
Publication statusPublished - 1999

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Galactosylceramidase
Larynx
Squamous Cell Carcinoma
Gene Expression
Keratinocytes
Genes
Mucous Membrane
Cell Line
5' Flanking Region
Gene Expression Profiling
Northern Blotting
Transfection
Exons
Carcinoma
Biopsy
Messenger RNA
Mutation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Transcriptional repression of the human galactocerebrosidase gene in squamous cell carcinomas of the larynx. / Görögh, Tibor; Rudert, Heinrich; Lippert, Burkard M.; Gottschlich, Stefan; Maune, Steffen; Heidorn, Klaus; Maass, Jan; Hoffmann, Markus; Meyer, Jens E.; Rathcke, Immo O.; Folz, Benedikt J.; Hortobágyi, T.; Werner, Jochen A.

In: International Journal of Cancer, Vol. 83, No. 6, 1999, p. 750-754.

Research output: Contribution to journalArticle

Görögh, T, Rudert, H, Lippert, BM, Gottschlich, S, Maune, S, Heidorn, K, Maass, J, Hoffmann, M, Meyer, JE, Rathcke, IO, Folz, BJ, Hortobágyi, T & Werner, JA 1999, 'Transcriptional repression of the human galactocerebrosidase gene in squamous cell carcinomas of the larynx', International Journal of Cancer, vol. 83, no. 6, pp. 750-754. https://doi.org/10.1002/(SICI)1097-0215(19991210)83:6<750::AID-IJC9>3.0.CO;2-V
Görögh, Tibor ; Rudert, Heinrich ; Lippert, Burkard M. ; Gottschlich, Stefan ; Maune, Steffen ; Heidorn, Klaus ; Maass, Jan ; Hoffmann, Markus ; Meyer, Jens E. ; Rathcke, Immo O. ; Folz, Benedikt J. ; Hortobágyi, T. ; Werner, Jochen A. / Transcriptional repression of the human galactocerebrosidase gene in squamous cell carcinomas of the larynx. In: International Journal of Cancer. 1999 ; Vol. 83, No. 6. pp. 750-754.
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