Transcriptional repression by MYB3R proteins regulates plant organ growth

Kosuke Kobayashi, Toshiya Suzuki, Eriko Iwata, Norihito Nakamichi, Takamasa Suzuki, Poyu Chen, Misato Ohtani, Takashi Ishida, Hanako Hosoya, Sabine Müller, Tünde Leviczky, Aladár Pettkõ-Szandtner, Zsuzsanna Darula, Akitoshi Iwamoto, Mika Nomoto, Yasuomi Tada, Tetsuya Higashiyama, Taku Demura, John H. Doonan, Marie Theres HauserKeiko Sugimoto, Masaaki Umeda, Zoltán Magyar, Lászlõ Bögre, Masaki Ito

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

In multicellular organisms, temporal and spatial regulation of cell proliferation is central for generating organs with defined sizes and morphologies. For establishing and maintaining the post-mitotic quiescent state during cell differentiation, it is important to repress genes with mitotic functions. We found that three of the Arabidopsis MYB3R transcription factors synergistically maintain G2/M-specific genes repressed in post-mitotic cells and restrict the time window of mitotic gene expression in proliferating cells. The combined mutants of the three repressor-type MYB3R genes displayed long roots, enlarged leaves, embryos, and seeds. Genome-wide chromatin immunoprecipitation revealed that MYB3R3 binds to the promoters of G2/M-specific genes and to E2F target genes. MYB3R3 associates with the repressor-type E2F, E2FC, and the RETINOBLASTOMA RELATED proteins. In contrast, the activator MYB3R4 was in complex with E2FB in proliferating cells. With mass spectrometry and pairwise interaction assays, we identified some of the other conserved components of the multiprotein complexes, known as DREAM/dREAM in human and flies. In plants, these repressor complexes are important for periodic expression during cell cycle and to establish a post-mitotic quiescent state determining organ size.

Original languageEnglish
Pages (from-to)1992-2007
Number of pages16
JournalEMBO Journal
Volume34
Issue number15
DOIs
Publication statusPublished - Aug 4 2015

Keywords

  • DREAM complex
  • G2/M phase
  • MYB transcription factors
  • cell cycle regulation
  • cell differentiation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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  • Cite this

    Kobayashi, K., Suzuki, T., Iwata, E., Nakamichi, N., Suzuki, T., Chen, P., Ohtani, M., Ishida, T., Hosoya, H., Müller, S., Leviczky, T., Pettkõ-Szandtner, A., Darula, Z., Iwamoto, A., Nomoto, M., Tada, Y., Higashiyama, T., Demura, T., Doonan, J. H., ... Ito, M. (2015). Transcriptional repression by MYB3R proteins regulates plant organ growth. EMBO Journal, 34(15), 1992-2007. https://doi.org/10.15252/embj.201490899