Transcriptional regulation of the bidirectional hydrogenase in the cyanobacterium Synechocystis 6803

Éva Kiss, P. Kós, I. Vass

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

To identify optimal conditions for renewable hydrogen production from sunlight and water we have studied transcriptional changes of the hoxEFUYH genes encoding the bidirectional hydrogenase in the cyanobacterium Synechocystis PCC 6803. Transcript abundance detection by real time polymerase chain reaction was supplemented with variable chlorophyll fluorescence measurements to monitor redox changes of the photosynthetic electron transport chain. Our main observations are: (i) abundance of hox transcripts decreases in the dark and recovers in the light. (ii) Inhibition of the Calvin cycle by glycolaldehyde suppresses hox gene transcription, which can be restored by the addition of electron transport inhibitors 3-(3,4-dichlorophenyl)-1,1-dimethylurea and dibromothymoquinone. (iii) The transcript levels of all hox genes are increased in anoxia, with additional induction of hoxEF in darkness or in the presence of dibromothymoquinone. (iv) Plastoquinone pool redox changes are not correlated with hox transcript level changes. (v) Changes in the transcript levels of lexA and sll0359 genes, encoding putative regulators of hox genes, are only partly correlated with transcript changes of hox genes under different conditions. Our data demonstrate a previously unrecognized light- and oxygen-dependent regulation of hox gene transcription in Synechocystis PCC 6803, which is related to photosynthetic electron transport and to unidentified oxygen and redox sensors. We also conclude that neither LexA nor Sll0359 are likely to be exclusive regulators of hox gene transcription.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalJournal of Biotechnology
Volume142
Issue number1
DOIs
Publication statusPublished - Jun 1 2009

Fingerprint

Synechocystis
Hydrogenase
Homeobox Genes
Cyanobacteria
Genes
Dibromothymoquinone
Transcription
Electron Transport
Oxidation-Reduction
Gene encoding
Regulator Genes
Plastoquinone
Diuron
Oxygen
Light
Sunlight
Polymerase chain reaction
Darkness
Photosynthesis
Chlorophyll

Keywords

  • Cyanobacterium
  • Electron transport
  • hox genes
  • Hydrogenase
  • Photosystem II
  • Synechocystis PCC 6803

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Transcriptional regulation of the bidirectional hydrogenase in the cyanobacterium Synechocystis 6803. / Kiss, Éva; Kós, P.; Vass, I.

In: Journal of Biotechnology, Vol. 142, No. 1, 01.06.2009, p. 31-37.

Research output: Contribution to journalArticle

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