Transcriptional control of Notch signaling by a HOX and a PBX/EXD protein during vulval development in C. elegans

K. Takács-Vellai, T. Vellai, Estella B. Chen, Yue Zhang, Frédéric Guerry, Michael J. Stern, Fritz Müller

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The Notch signaling pathway controls growth, differentiation and patterning in divergent animal phyla; in humans, defective Notch signaling has been implicated in cancer, stroke and neurodegenerative disorders. Despite its developmental and medical significance, little is known about the factors that render cells to become competent for Notch signaling. Here we show that during vulval development in the nematode Caenorhabditis elegans the HOX protein LIN-39 and its EXD/PBX-like cofactor CEH-20 are required for LIN-12/Notch-mediated lateral signaling that specifies the 2° vulval cell fate. Inactivation of either lin-39 or ceh-20 resulted in the misspecification of 2° vulval cells and suppressed the multivulva phenotype of lin-12(n137) gain-of-function mutant animals. Furthermore, both LIN-39 and CEH-20 are required for the expression of basal levels of the genes encoding the LIN-12/Notch receptor and one of its ligands in the vulval precursor cells, LAG-2/Delta/Serrate, rendering them competent for the subsequent lin-12/Notch induction events. Our results suggest that the transcription factors LIN-39 and CEH-20, which function at the bottom of the RTK/Ras and Wnt pathways in vulval induction, serve as major integration sites in coordinating and transmitting signals to the LIN-12/Notch cascade to regulate vulval cell fates.

Original languageEnglish
Pages (from-to)661-669
Number of pages9
JournalDevelopmental Biology
Volume302
Issue number2
DOIs
Publication statusPublished - Feb 15 2007

Fingerprint

Proteins
Caenorhabditis elegans Proteins
Notch Receptors
Wnt Signaling Pathway
Neurodegenerative Diseases
Transcription Factors
Stroke
dixanthogen
Ligands
Phenotype
Growth
Genes
Neoplasms

Keywords

  • C. elegans
  • CEH-20/PBX/EXD
  • LAG-2/Delta ligand
  • LIN-12 receptor
  • LIN-39/HOX
  • Notch signaling
  • Signaling crosstalk
  • Vulval induction

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Transcriptional control of Notch signaling by a HOX and a PBX/EXD protein during vulval development in C. elegans. / Takács-Vellai, K.; Vellai, T.; Chen, Estella B.; Zhang, Yue; Guerry, Frédéric; Stern, Michael J.; Müller, Fritz.

In: Developmental Biology, Vol. 302, No. 2, 15.02.2007, p. 661-669.

Research output: Contribution to journalArticle

Takács-Vellai, K. ; Vellai, T. ; Chen, Estella B. ; Zhang, Yue ; Guerry, Frédéric ; Stern, Michael J. ; Müller, Fritz. / Transcriptional control of Notch signaling by a HOX and a PBX/EXD protein during vulval development in C. elegans. In: Developmental Biology. 2007 ; Vol. 302, No. 2. pp. 661-669.
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