Transcription specificity of the class Ib genes SLA-6, SLA-7 and SLA-8 of the swine major histocompatibility complex and comparison with class Ia genes

S. Kusza, L. Flori, Y. Gao, A. Teillaud, R. Hu, G. Lemonnier, Z. Bösze, E. Bourneuf, S. Vincent-Naulleau, C. Rogel-Gaillard

Research output: Contribution to journalArticle

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Abstract

Our aim was to analyse the transcription levels of the three non-classical class Ib genes SLA-6, SLA-7 and SLA-8 of the swine major histocompatibility complex in various tissues and conditions and to compare them to the transcription levels of classical class Ia genes. Twenty-five adult tissues from two pig breeds, pig renal PK15 cells infected with the Pseudorabies virus, and peripheral blood mononuclear cells (PBMCs) stimulated by lipopolysaccharide or a mixture of phorbol myristate acetate and ionomycin were included in our study. Relative transcription was quantified by quantitative real-time PCR. On average, in adult tissues and PBMCs and compared to SLA-6, the transcription level of SLA-Ia genes was 100-1000 times higher, the level of SLA-8 was 10-20 times higher, and that of SLA-7 was five times higher. Thus, SLA-8 is the most transcribed SLA-Ib gene, followed by the SLA-7 and SLA-6 genes. The highest transcription levels of SLA-Ib transcripts were found in the lymphoid organs, followed by the lung and the digestive tract. The tissue variability of expression levels was widest for the SLA-6 gene, with a 1:32 ratio between the lowest and highest levels in contrast to a 1:12 ratio for the SLA-7 and SLA-8 genes and a 1:16 ratio for the SLA-Ia genes. During PK-15 infection and PBMC stimulation, SLA-Ia and SLA-8 genes were downregulated, whereas SLA-6 and SLA-7 were upregulated, downregulated or not significantly modified. Our overall results confirm the tissue-wide transcription of the three SLA-Ib genes and suggest that they have complementary roles.

Original languageEnglish
Pages (from-to)510-520
Number of pages11
JournalAnimal Genetics
Volume42
Issue number5
DOIs
Publication statusPublished - Oct 2011

Fingerprint

major histocompatibility complex
Major Histocompatibility Complex
Swine
transcription (genetics)
swine
Genes
genes
mononuclear leukocytes
Blood Cells
Down-Regulation
Suid Herpesvirus 1
Ionomycin
swine breeds
Suid herpesvirus 1
Tetradecanoylphorbol Acetate
kidney cells
digestive tract
lipopolysaccharides
Lipopolysaccharides
Gastrointestinal Tract

Keywords

  • class Ia
  • class Ib
  • major histocompatibility complex
  • pig
  • SLA-6
  • SLA-7
  • SLA-8
  • transcription

ASJC Scopus subject areas

  • Genetics
  • Animal Science and Zoology

Cite this

Transcription specificity of the class Ib genes SLA-6, SLA-7 and SLA-8 of the swine major histocompatibility complex and comparison with class Ia genes. / Kusza, S.; Flori, L.; Gao, Y.; Teillaud, A.; Hu, R.; Lemonnier, G.; Bösze, Z.; Bourneuf, E.; Vincent-Naulleau, S.; Rogel-Gaillard, C.

In: Animal Genetics, Vol. 42, No. 5, 10.2011, p. 510-520.

Research output: Contribution to journalArticle

Kusza, S, Flori, L, Gao, Y, Teillaud, A, Hu, R, Lemonnier, G, Bösze, Z, Bourneuf, E, Vincent-Naulleau, S & Rogel-Gaillard, C 2011, 'Transcription specificity of the class Ib genes SLA-6, SLA-7 and SLA-8 of the swine major histocompatibility complex and comparison with class Ia genes', Animal Genetics, vol. 42, no. 5, pp. 510-520. https://doi.org/10.1111/j.1365-2052.2010.02170.x
Kusza, S. ; Flori, L. ; Gao, Y. ; Teillaud, A. ; Hu, R. ; Lemonnier, G. ; Bösze, Z. ; Bourneuf, E. ; Vincent-Naulleau, S. ; Rogel-Gaillard, C. / Transcription specificity of the class Ib genes SLA-6, SLA-7 and SLA-8 of the swine major histocompatibility complex and comparison with class Ia genes. In: Animal Genetics. 2011 ; Vol. 42, No. 5. pp. 510-520.
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AU - Gao, Y.

AU - Teillaud, A.

AU - Hu, R.

AU - Lemonnier, G.

AU - Bösze, Z.

AU - Bourneuf, E.

AU - Vincent-Naulleau, S.

AU - Rogel-Gaillard, C.

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