Transcription of the Arabidopsis CPD gene, encoding a steroidogenic cytochrome P450, is negatively controlled by brassinosteroids

Jaideep Mathur, Gergely Molnár, Shozo Fujioka, Suguru Takatsuto, Akira Sakurai, Takao Yokota, Günter Adam, Brunhilde Voigt, Ferenc Nagy, Christoph Maas, Jeff Schell, Csaba Koncz, Miklós Szekeres

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Abstract

The Arabidopsis CPD gene encodes a cytochrome P450 steroid side-chain hydroxylase (CYP90) that plays an essential role in the biosynthesis of the plant hormone brassinolide. Expression of the CPD gene is confined to cotyledons and leaf primordia in etiolated seedlings and detectable in the adaxial parenchyma of expanding leaves in light-grown plants. Transcription of the CPD gene is not affected by the plant growth factors auxin, ethylene, gibberellin, cytokinin, jasmonic acid and salicylic acid, but is specifically down-regulated by brassinolide in both dark and light. Steady-state mRNA levels of a CPD promoter-driven uidA reporter gene correlate with the expression of resident CPD gene in transgenic plants. Intermediates of the early and late C-6 oxidation pathways of brassinolide, carrying C-22 and C-23 side-chain hydroxyls, efficiently inhibit the activity of the CPD promoter. Repression of CPD transcription by brassinosteroids is sensitive to the protein synthesis inhibitor cycloheximide, indicating a requirement for de novo synthesis of a regulatory factor.

Original languageEnglish
Pages (from-to)593-602
Number of pages10
JournalPlant Journal
Volume14
Issue number5
DOIs
Publication statusPublished - Jun 1 1998

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ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Mathur, J., Molnár, G., Fujioka, S., Takatsuto, S., Sakurai, A., Yokota, T., Adam, G., Voigt, B., Nagy, F., Maas, C., Schell, J., Koncz, C., & Szekeres, M. (1998). Transcription of the Arabidopsis CPD gene, encoding a steroidogenic cytochrome P450, is negatively controlled by brassinosteroids. Plant Journal, 14(5), 593-602. https://doi.org/10.1046/j.1365-313X.1998.00158.x