Transcription of cauliflower mosaic virus DNA: detection of promoter sequences, and characterization of transcripts

Hubert Guilley, Roderick K. Dudley, Gérard Jonard, E. Balázs, Kenneth E. Richards

Research output: Contribution to journalArticle

221 Citations (Scopus)

Abstract

Four RNA transcripts encoded by cauliflower mosaic virus DNA have been detected in the polyadenylated RNA from virus-infected turnip leaves. Two of these transcripts, the major 35S and the 8S species, have the same 5′ termini, at nucleotide 7435. A viral DNA fragment encompassing this region directs transcription initiation at this point in vitro. The 5′ terminus of the 19S transcript is at nucleotide 5764, and a corresponding viral DNA fragment also directs transcription initiation in vitro. The major 35S RNA is a complete transcript of the circular viral genome, and is 3′-coterminal with 19S RNA at nucleotide 7615. The 8S RNA has its 3′ extremity at Δ1, the single-stranded interruption in the transcribed strand of virion DNA. A minor 35S RNA has also been detected that has its 5′ and 3′ termini at Δ1.

Original languageEnglish
Pages (from-to)763-773
Number of pages11
JournalCell
Volume30
Issue number3
DOIs
Publication statusPublished - 1982

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Caulimovirus
Transcription
Viruses
RNA
DNA
Nucleotides
Viral DNA
Brassica napus
Viral Genome
RNA Viruses
Virion
Extremities
Genes
Messenger RNA

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Transcription of cauliflower mosaic virus DNA : detection of promoter sequences, and characterization of transcripts. / Guilley, Hubert; Dudley, Roderick K.; Jonard, Gérard; Balázs, E.; Richards, Kenneth E.

In: Cell, Vol. 30, No. 3, 1982, p. 763-773.

Research output: Contribution to journalArticle

Guilley, Hubert ; Dudley, Roderick K. ; Jonard, Gérard ; Balázs, E. ; Richards, Kenneth E. / Transcription of cauliflower mosaic virus DNA : detection of promoter sequences, and characterization of transcripts. In: Cell. 1982 ; Vol. 30, No. 3. pp. 763-773.
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