Transcription factor RFX1 helps control the promoter of the mouse ribosomal protein-encoding gene rpL30 by binding to its α element

Geza Sáfrány, Robert P. Perry

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The factor that binds to the most upstream element (α) of the mouse rpL30 promoter was identified as RFX1, a novel 105-kDa protein that recognizes an important element of MHC class-II promoters. Identification was based on competition between rpL30α. and an RFX1-binding site for nuclear protein complex formation and on the ability of RFX1 antibody to supershift the electrophoretic mobility of the DNA-protein complexes. A mutation in the α-element that abolished its interaction with RFX1 reduced rpL30 promoter activity to about 43% of the wild-type level, indicating that RFX1 plays an important role in determining the strength of the rpL30 promoter. A search of a eukaryotic promoter database revealed candidate RFX1-binding sites in a variety of other promoters, suggesting that this protein may be implicated in the transcriptional regulation of a wide variety of genes.

Original languageEnglish
Pages (from-to)279-283
Number of pages5
JournalGene
Volume132
Issue number2
DOIs
Publication statusPublished - Oct 15 1993

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Keywords

  • MHC class-II gene
  • Regulatory promoter element
  • electrophoretic supershift

ASJC Scopus subject areas

  • Genetics

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