Transcript identification and profiling during salt stress and recovery of Populus euphratica

Ruisheng Gu, Sandra Fonseca, L. Puskás, László Hackler, A. Zvara, D. Dudits, Maria S. Pais

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Populus euphratica Oli. is a salt-tolerant species that can cope with up to 450 mM NaCl under hydroponic conditions and can tolerate high accumulations of Na+ and Cl- in roots and leaves when grown in 300 mM NaCl. Transcript responses to salt stress and recovery were monitored by microarray hybridization of 315 cDNAs preselected by suppression subtractive hybridization. Transcripts of a heat-shock protein and a hydroxyproline-rich glycoprotein accumulated 1.5 and 3 h, respectively, after adding 300 mM NaCl to the culture medium. Transcripts significantly up-regulated by salt stress included ionic and osmotic homeostasis elements such as magnesium transporter-like protein, syntaxin-like protein, seed imbibition protein and plasma membrane intrinsic protein; metabolism regulators like cytochrome P450, zinc finger protein, cleavage factor and aminotransferase; and the photosynthesis-activating enzyme Rubisco activase and photorespiration-related glycolate oxidase. Several photosynthesis-related transcripts were down-regulated in response to 72 h of salt stress but were up-regulated after long-term recovery (48 h). Sucrose synthase, ABC transporter, calmodulin, Pop3 peptide and aquaporin appeared to be actively involved in the process of plant recovery from salt stress. Several transcripts encoding proteins of unknown function were regulated by salt stress. Selected transcripts exhibiting altered transcript profiles in response to salt stress were also analyzed by real-time quantitative PCR. Transcript analysis during salt stress and recovery of this woody species revealed several genes and corresponding proteins deserving special attention in future studies of salt tolerance in woody species.

Original languageEnglish
Pages (from-to)265-276
Number of pages12
JournalTree Physiology
Volume24
Issue number3
Publication statusPublished - Mar 2004

Fingerprint

Populus euphratica
Populus
salt stress
Salts
salt
protein
proteins
Photosynthesis
Proteins
photosynthesis
(S)-2-hydroxy-acid oxidase
microarray technology
Qa-SNARE Proteins
Hydroponics
suppression subtractive hybridization
ABC transporters
Ribulose-Bisphosphate Carboxylase
sucrose synthase
aquaporins
photorespiration

Keywords

  • Ionic intensity
  • Ionic mapping
  • Microarray
  • Realtime PCR
  • Suppression subtractive hybridization
  • Transcript regulation

ASJC Scopus subject areas

  • Forestry
  • Plant Science

Cite this

Transcript identification and profiling during salt stress and recovery of Populus euphratica. / Gu, Ruisheng; Fonseca, Sandra; Puskás, L.; Hackler, László; Zvara, A.; Dudits, D.; Pais, Maria S.

In: Tree Physiology, Vol. 24, No. 3, 03.2004, p. 265-276.

Research output: Contribution to journalArticle

Gu, Ruisheng ; Fonseca, Sandra ; Puskás, L. ; Hackler, László ; Zvara, A. ; Dudits, D. ; Pais, Maria S. / Transcript identification and profiling during salt stress and recovery of Populus euphratica. In: Tree Physiology. 2004 ; Vol. 24, No. 3. pp. 265-276.
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