Trace amounts of Triton X-100 modify the inhibitor sensitivity of the mitochondrial porin

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Abstract

Transport properties of mitochondrial porin were investigated on the basis of changes in the activity of hexokinase utilizing external ATP. Production of glucose: 6-phosphate is inhibited by polyanion both in intact brain mitochondria and in contact point vesicles. Hexokinase activity is restored by solubilization of the enzyme by high ionic strength or 0.5-1% Triton X-100. In very low concentrations (0.001-0.005%) Triton does not mobilize hexokinase from its binding sites but it is able to release polyanion-inhibition completely. This finding provides an explanation for the discrepancy observed in the transport properties of porin when studied 'in situ' or in artificial lipid membranes.

Original languageEnglish
Pages (from-to)249-254
Number of pages6
JournalBBA - Biomembranes
Volume1234
Issue number2
DOIs
Publication statusPublished - Mar 22 1995

Keywords

  • Contact point
  • Hexokinase
  • Polyanion
  • Porin
  • Triton
  • VDAC

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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