Toxicity of cylindrospermopsin to the brine shrimp Artemia salina

Comparisons with protein synthesis inhibitors and microcystins

J. S. Metcalf, J. Lindsay, K. A. Beattie, S. Birmingham, M. L. Saker, A. Törökné, G. A. Codd

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

The Artemia salina bioassay was successfully applied to the analysis of the hepatotoxic cyanobacterial alkaloid and protein synthesis inhibitor, cylindrospermopsin. A dose-dependent response in mortality was observed for purified cylindrospermopsin and LC50 values decreased with time from 8.1 to 0.71μgml-1, between 24 and 72h, respectively. Cylindrospermopsin was slightly less potent than microcystin-LR, with similar LC50 values on a gravimetric basis, but was more toxic to A.salina than the protein synthesis inhibitors, cycloheximide, chloramphenicol and tetracycline. Cylindrospermopsin-containing strains of the cyanobacterium Cylindrospermopsis raciborskii were found to be toxic to A.salina and the LC50 concentration for these strains over time was greater than the LC50 for purified cylindrospermopsin, with the exception of C. raciborskii strain CR1.

Original languageEnglish
Pages (from-to)1115-1120
Number of pages6
JournalToxicon
Volume40
Issue number8
DOIs
Publication statusPublished - 2002

Fingerprint

Microcystins
Artemia
Protein Synthesis Inhibitors
Toxicity
Poisons
Cylindrospermopsis
Bioassay
Cyanobacteria
Chloramphenicol
Cycloheximide
Tetracycline
Alkaloids
Biological Assay
Salinum
cylindrospermopsin
brine
Mortality

Keywords

  • Artemia
  • Cyanobacteria
  • Cylindrospermopsin
  • Cylindrospermopsis
  • Microcystin

ASJC Scopus subject areas

  • Toxicology

Cite this

Toxicity of cylindrospermopsin to the brine shrimp Artemia salina : Comparisons with protein synthesis inhibitors and microcystins. / Metcalf, J. S.; Lindsay, J.; Beattie, K. A.; Birmingham, S.; Saker, M. L.; Törökné, A.; Codd, G. A.

In: Toxicon, Vol. 40, No. 8, 2002, p. 1115-1120.

Research output: Contribution to journalArticle

Metcalf, J. S. ; Lindsay, J. ; Beattie, K. A. ; Birmingham, S. ; Saker, M. L. ; Törökné, A. ; Codd, G. A. / Toxicity of cylindrospermopsin to the brine shrimp Artemia salina : Comparisons with protein synthesis inhibitors and microcystins. In: Toxicon. 2002 ; Vol. 40, No. 8. pp. 1115-1120.
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