Toxic Oxygen Metabolites and Ischemia-Reperfusion Increase Histamine Synthesis and Release in the Isolated Rat Heart

G. Valen, J. Kaszaki, I. Szabó, S. Nagy, J. Vaage

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Histamine is synthetized in the heart, and released by ischemia-reperfusion injury in several species. Histamine has arrhythmogenic, chronotropic, inotropic and vasoactive effects. Cardiac histamine release during ischemia-reperfusion may be mediated by toxic oxygen metabolites. We studied the effect of ischemia-reperfusion and toxic oxygen metabolites on release and synthesis of histamine in the isolated rat heart (Langendorff model). The following groups were included: I, (n=10) control perfusion for 60 min; II, (n=7) H2O2 (200 μM) was given for 10 min followed by 50 min recovery; III, (n=7) thiourea (15 mM) was given in addition to H2O2; IV, (n=7) thiourea given alone; V, (n=7) catalase (150 U/ml) plus H2O2); VI, (n=7) 20 min ischemia followed by 40 min reperfusion. The contents of histamine in the coronary effluent and in cardiac tissue were measured repeatedly (radioenzymatic method). Ischemia-reperfusion and toxic oxygen metabolites increased release of histamine in the coronary effluent. Concomitantly the histamine contents in cardiac tissue increased, indicating increased synthesis of histamine.

Original languageEnglish
Pages (from-to)31-40
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume25
Issue number1
DOIs
Publication statusPublished - Jan 1993

Fingerprint

Histamine Release
Poisons
Histamine
Reperfusion
Ischemia
Oxygen
Thiourea
Reperfusion Injury
Catalase
Perfusion

Keywords

  • Catalase
  • Histamine
  • Hydrogen peroxide
  • Ischemia-reperfusion
  • Rat
  • Thiourea
  • Toxic oxygen metabolites

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Molecular Biology

Cite this

Toxic Oxygen Metabolites and Ischemia-Reperfusion Increase Histamine Synthesis and Release in the Isolated Rat Heart. / Valen, G.; Kaszaki, J.; Szabó, I.; Nagy, S.; Vaage, J.

In: Journal of Molecular and Cellular Cardiology, Vol. 25, No. 1, 01.1993, p. 31-40.

Research output: Contribution to journalArticle

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