Towards the production of stress tolerant grapevine cultivars

A. Zok, R. Oláh, Gy Váradi, E. Szegedi, B. P. Kós, É Hideg, V. G. Horváth, B. Báló

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Abstract

The biotic and abiotic stresses are among the major limiting factors in plant productivity. To overcome these difficulties molecular breeding methods have recently been widely applied to improve the stress-and disease resistance of grapevine cultivars. To this end we tested the suitability of a ferritin gene derived from Medicago sativa (MsFerr). We propose that by sequestering the intracellular iron involved in generation of the very reactive hydroxyl radicals through Fentonreaction, the increased overall ferritin concentration results in increased protection of plant cells from oxidative damage induced by a wide range of stresses. We have improved the existing regeneration and transformation protocols. In our experiments we raised the number of regenerated plants due to the application of 1 μM benzyladenine (BA). The plant regeneration was supported by the maintenance of plant material on "half MS" medium without selection agent (after two-year selection), and by cut of abnormal embryos under the hypocotyl. The independent regenerated transformants were tested by PCR, qPCR, Western blot, and were used for various abiotic stress tolerance experiments.

Original languageEnglish
Title of host publicationActa Horticulturae
Pages651-657
Number of pages7
Publication statusPublished - Dec 1 2009

Publication series

NameActa Horticulturae
Volume839
ISSN (Print)0567-7572

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Keywords

  • Ferritin
  • Genetic transformation
  • Oxidative damages
  • Regeneration
  • Somatic embryos

ASJC Scopus subject areas

  • Horticulture

Cite this

Zok, A., Oláh, R., Váradi, G., Szegedi, E., Kós, B. P., Hideg, É., Horváth, V. G., & Báló, B. (2009). Towards the production of stress tolerant grapevine cultivars. In Acta Horticulturae (pp. 651-657). (Acta Horticulturae; Vol. 839).