Towards the map based cloning of a recessive Fusarium resistance determinant from diploid Medicago sativa

Márta Balogh, Krisztina Miró, Ágnes Dalmadi, Góbor Deák, Tünde Petrovics, Brigitta Dudás, Péter Kiss, Júlia Jakab, György Botond Kiss

Research output: Contribution to journalArticle

Abstract

A monogenic recessive Fusarium resistance determinant was identified in a diploid Medicago sativa population. The Fusarium susceptible and resistant phenotypes of the individuals in an F2 segregation population was inferred from a biological test using injured root test. The resulting phenotypes were used to position the Fusarium resistance determinant on linkage group 6 of the Medicago sativa genetic map. Using the DNA based molecular markers genome walk was initiated from both sides of the genetic region constructing two contigs which are about 0.5 centiMorgan (500 kilobasepairs) apart. The introduction of this genetic determinant into tetraploid alfalfa cultivars was unsuccessful since only triploid and pentaploid hybrids could be recovered from the 4-2 crosses. The isolation of the gene and establishment of the function of this recessive Fusarium determinant is under progress.

Original languageEnglish
Pages (from-to)279-289
Number of pages11
JournalActa Phytopathologica et Entomologica Hungarica
Volume42
Issue number2
DOIs
Publication statusPublished - Dec 1 2007

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Keywords

  • Fusarium resistance
  • Genetic mapping
  • Map based cloning
  • Medicago
  • Pathogen interaction

ASJC Scopus subject areas

  • Plant Science
  • Insect Science

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