Towards the development of a simplified long-term organ culture method for human scalp skin and its appendages under serum-free conditions

Zhongfa Lu, Sybille Hasse, E. Bódó, Christian Rose, Wolfgang Funk, Ralf Paus

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Organ culture of human scalp skin is usually performed with serum-containing medium, which limits its analytical usefulness. Here we report that intact human scalp skin can be grown at the air/liquid interface in supplemented, serum-free William's E medium for more than 2 weeks. Active hair shaft growth was visible until day 16 and was significantly enhanced compared with minimum essential medium (MEM)+10% fetal bovine serum (FBS). Moreover, William's E medium protected better against cell death than MEM + 10% FBS before day 12. Using quantitative immunochemistry, proliferating (Ki-67+) cells could still be observed in the epithelium of hair follicles even on day 17 of serum-free skin organ culture. The number of apoptotic (TUNEL+) cells in the skin epithelium rose steadily after day 5. Giemsa stains revealed mature skin mast cells even after 13 days in culture. The percentage of surviving hair follicles (mostly with catagen- or telogen-like morphology) gradually increased over time displaying mostly catagen hair follicles after 17 days of culture. Although epidermis and hair follicle epithelium showed increasing atrophy and degeneration, and their pigmentation decreased gradually over time, some long-term-surviving epithelial islands were found in association with remnants of follicular structures as late as on day 88. These preliminary data suggest that a very simple serum-free organ culture method allows prolonged human skin and hair follicle survival as well as some limited hair follicle cycling in intact skin for more than 2 weeks under well-defined experimental conditions. This pragmatic assay invites multiple uses, and may become a valuable tool for both skin and hair research.

Original languageEnglish
Pages (from-to)37-44
Number of pages8
JournalExperimental Dermatology
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 2007

Fingerprint

Organ Culture Techniques
Scalp
Hair Follicle
Skin
Serum
Epithelium
Hair
Azure Stains
Immunochemistry
In Situ Nick-End Labeling
Pigmentation
Cell death
Islands
Epidermis
Mast Cells
Atrophy
Assays
Cell Death
Air
Association reactions

Keywords

  • Hair follicle
  • Hair growth
  • Human scalp skin
  • Long-term organ culture
  • Pigmentation

ASJC Scopus subject areas

  • Dermatology

Cite this

Towards the development of a simplified long-term organ culture method for human scalp skin and its appendages under serum-free conditions. / Lu, Zhongfa; Hasse, Sybille; Bódó, E.; Rose, Christian; Funk, Wolfgang; Paus, Ralf.

In: Experimental Dermatology, Vol. 16, No. 1, 01.2007, p. 37-44.

Research output: Contribution to journalArticle

Lu, Zhongfa ; Hasse, Sybille ; Bódó, E. ; Rose, Christian ; Funk, Wolfgang ; Paus, Ralf. / Towards the development of a simplified long-term organ culture method for human scalp skin and its appendages under serum-free conditions. In: Experimental Dermatology. 2007 ; Vol. 16, No. 1. pp. 37-44.
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