Towards objective hand hygiene technique assessment: validation of the ultraviolet-dye-based hand-rubbing quality assessment procedure

Lehotsky, L. Szilágyi, S. Bánsághi, P. Szerémy, G. Wéber, T. Haidegger

Research output: Contribution to journalArticle

3 Citations (Scopus)


Ultraviolet spectrum markers are widely used for hand hygiene quality assessment, although their microbiological validation has not been established. A microbiology-based assessment of the procedure was conducted. Twenty-five artificial hand models underwent initial full contamination, then disinfection with UV-dyed hand-rub solution, digital imaging under UV-light, microbiological sampling and cultivation, and digital imaging of the cultivated flora were performed. Paired images of each hand model were registered by a software tool, then the UV-marked regions were compared with the pathogen-free sites pixel by pixel. Statistical evaluation revealed that the method indicates correctly disinfected areas with 95.05% sensitivity and 98.01% specificity.

Original languageEnglish
Pages (from-to)26-29
Number of pages4
JournalJournal of Hospital Infection
Issue number1
Publication statusPublished - Sep 1 2017



  • Hand hygiene assessment
  • Infection control
  • Infection prevention
  • UV-dyed hand rub
  • Validation

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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