Toward the generation of an aminonaphthalene trisulfonate labeled N-glycan database for capillary gel electrophoresis analysis of carbohydrates

Márta Kerékgyártó, A. Guttman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

There is an increasing trend to develop therapeutic glycoproteins, mostly antibodies that require high resolution bioanalytical tools to address the challenging aspects of comprehensive carbohydrate characterization. In this paper we introduce an initial version of a glucose unit database for 8-aminonaphthalene-1,3,6-trisulfonic acid-labeled glycans. At this stage we mainly focused on therapeutic IgG derived glycans of core fucosylated biantennary structures with and without sialic acid residues, as well as high mannose structures. Currently 25 oligosaccharides represent this first set of the database that shows the abbreviated names of the individual sugar structures with their graphic representation, precise molecular mass and glucose unit (GU) values with corresponding SDs. The database will provide a quick glycan analysis tool for preliminary data interpretation of rapid (around 200 s) CGE-LED-induced fluorescence (CGE-LEDIF) based glycan profiling runs.

Original languageEnglish
Pages (from-to)2222-2228
Number of pages7
JournalElectrophoresis
Volume35
Issue number15
DOIs
Publication statusPublished - 2014

Fingerprint

Capillary Electrophoresis
Electrophoresis
Polysaccharides
Gels
Carbohydrates
Databases
1-Naphthylamine
Glucose
Molecular mass
N-Acetylneuraminic Acid
Mannose
Oligosaccharides
Sugars
Names
Light emitting diodes
Glycoproteins
Immunoglobulin G
Fluorescence
Acids
Antibodies

Keywords

  • ANTS labeling
  • CGE
  • Database
  • Glycan analysis
  • Structural elucidation

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Medicine(all)

Cite this

Toward the generation of an aminonaphthalene trisulfonate labeled N-glycan database for capillary gel electrophoresis analysis of carbohydrates. / Kerékgyártó, Márta; Guttman, A.

In: Electrophoresis, Vol. 35, No. 15, 2014, p. 2222-2228.

Research output: Contribution to journalArticle

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