Topographical distribution of pro-opiomelanocortin-derived peptides (ACTH/β-END/α-MSH) in the rat median eminence

J. Kiss, E. Mezey, M. D. Cassell, T. H. Williams, G. P. Mueller, T. L. O'Donohue, M. Palkóvits

Research output: Contribution to journalArticle

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Abstract

The detailed distribution of adrenocorticotropin (ACTH), β-endorphin (β-END) and α-melanotropin (α-MSH) immunoreactivity was examined in the rat median eminence (ME) and pituitary stalk using light microscopic immunocytochemistry and radioimmunoassay (RIA). Nerve fibers and varicosities immunoreactive for ACTH/β-END/α-MSH had identical distributions in the ME suggesting that they are part of the same arcuate proopiomelanocortin neuronal (POMC) system. The quantitative image analysis of POMC immunoreactive varicosities in the ME indicates no significant differences between the various rostro-caudal segments. In the main (preinfundibular) portion of the ME, a moderate density of immunoreactive elements was located in the lateral part of the internal zone and throughout the postinfundibular ME. Very few scattered varicosities were observed in the neurohemal (external) zone and in the pituitary stalk. By RIA, α-MSH is present in a substantially higher concentration than ACTH and β-END throughout the ME. Knife cuts between the arcuate nucleus and ME indicate that proopiomelanocortin (POMC) fibers enter the ME in its whole rostro-caudal extent. Thus POMC neurons seem to provide innervation of structures in the internal zone but not in the neurohemal/external/zone where the portal capillary system is located. Moreover, the observation that the density of immunoreactive elements is substantially lower in the pituitary stalk than in the ME, suggests that the majority of immunoreactive fibers in the internal zone are not fibers of passage directed towards the neurohypophysis.

Original languageEnglish
Pages (from-to)169-176
Number of pages8
JournalBrain Research
Volume329
Issue number1-2
DOIs
Publication statusPublished - Mar 11 1985

Fingerprint

Melanocyte-Stimulating Hormones
Pro-Opiomelanocortin
Median Eminence
Adrenocorticotropic Hormone
Peptides
Pituitary Gland
Radioimmunoassay
Endorphins
Portal System
Posterior Pituitary Gland
Arcuate Nucleus of Hypothalamus
Nerve Fibers
Immunohistochemistry
Neurons
Light

Keywords

  • adrenocorticotropin
  • immunocytochemistry
  • median eminence
  • quantitative image analysis
  • radioimmunoassay
  • α-melanotropin
  • β-endorphin

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Topographical distribution of pro-opiomelanocortin-derived peptides (ACTH/β-END/α-MSH) in the rat median eminence. / Kiss, J.; Mezey, E.; Cassell, M. D.; Williams, T. H.; Mueller, G. P.; O'Donohue, T. L.; Palkóvits, M.

In: Brain Research, Vol. 329, No. 1-2, 11.03.1985, p. 169-176.

Research output: Contribution to journalArticle

Kiss, J. ; Mezey, E. ; Cassell, M. D. ; Williams, T. H. ; Mueller, G. P. ; O'Donohue, T. L. ; Palkóvits, M. / Topographical distribution of pro-opiomelanocortin-derived peptides (ACTH/β-END/α-MSH) in the rat median eminence. In: Brain Research. 1985 ; Vol. 329, No. 1-2. pp. 169-176.
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AU - Cassell, M. D.

AU - Williams, T. H.

AU - Mueller, G. P.

AU - O'Donohue, T. L.

AU - Palkóvits, M.

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AB - The detailed distribution of adrenocorticotropin (ACTH), β-endorphin (β-END) and α-melanotropin (α-MSH) immunoreactivity was examined in the rat median eminence (ME) and pituitary stalk using light microscopic immunocytochemistry and radioimmunoassay (RIA). Nerve fibers and varicosities immunoreactive for ACTH/β-END/α-MSH had identical distributions in the ME suggesting that they are part of the same arcuate proopiomelanocortin neuronal (POMC) system. The quantitative image analysis of POMC immunoreactive varicosities in the ME indicates no significant differences between the various rostro-caudal segments. In the main (preinfundibular) portion of the ME, a moderate density of immunoreactive elements was located in the lateral part of the internal zone and throughout the postinfundibular ME. Very few scattered varicosities were observed in the neurohemal (external) zone and in the pituitary stalk. By RIA, α-MSH is present in a substantially higher concentration than ACTH and β-END throughout the ME. Knife cuts between the arcuate nucleus and ME indicate that proopiomelanocortin (POMC) fibers enter the ME in its whole rostro-caudal extent. Thus POMC neurons seem to provide innervation of structures in the internal zone but not in the neurohemal/external/zone where the portal capillary system is located. Moreover, the observation that the density of immunoreactive elements is substantially lower in the pituitary stalk than in the ME, suggests that the majority of immunoreactive fibers in the internal zone are not fibers of passage directed towards the neurohypophysis.

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