Topical acetone treatment induces neurogenic oedema on the sensitized mouse ear: An in vivo study using transient receptor potential vanilloid 1 (TRPV1) receptor knockout mice

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Objective: The participation of sensory neurons and transient receptor potential vanilloid 1 (TRPV1) receptors in phorbol 12-myristate 13-acetate (PMA)-induced nerve-sensitizing effect was examined. Materials and methods: PMA dissolved in acetone and acetone were applied to the ears of TRPV1 receptor knockout and wild-type mice. Different groups of animals received ibuprofen, anti-interleukin-1 beta (IL-1β) antibody, resiniferatoxin (RTX) or capsaicin pretreatment. Ear thickness, myeloperoxidase activity and IL-1β content of the ears were determined. Histological evaluation was performed. Results: PMA exerted potentiating action on contralateral acetone-induced ear oedema, which was inhibited by ibuprofen, topical capsaicin desensitization of the acetone-treated ear as well as by systemic RTX pretreatment. Neither the lack of TRPV1 receptors nor anti-IL-1β antibody prevented sensitizing effect. Conclusions: The TRPV1 receptor-independent potentiating action of PMA on contralateral acetone-induced ear oedema is mediated via capsaicin-sensitive afferents and prostanoids are involved. IL-1β is not essential in this process.

Original languageEnglish
Pages (from-to)459-467
Number of pages9
JournalInflammation Research
Issue number11
Publication statusPublished - Nov 1 2007



  • IL-1β
  • Phorbol 12-myristate 13-acetate
  • Prostanoids
  • Resiniferatoxin
  • TRPV1

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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