Tools for flash-photolysis experiments on voltage-clamped muscle fibre segments

R. P. Schuhmeier, S. Tewes, P. Szentesi, W. Melzer

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

An experimental set-up is described that allows the combination of rapid transmembrane voltage changes and photometric calcium recording with the fast photochemical turnover of substances applied externally or intracellularly to cut skeletal muscle fibres. It consists of a double-vaseline-gap system, designed for use with a xenon-flash-lamp device and a dual-wavelength microscope photometer. The pools of the vaseline gap chamber that contain the solutions surrounding the cut ends and the voltage-clamped segment of the muscle fibre are closed and have volumes of 20-50 μl. Thin tubes allow rapid solution change or continuous perfusion in the chamber compartments. Accessory tools were constructed to simplify focussing and measuring the flash-light intensity. A pilot light delivered from a red laser diode is used as a guide beam to target the ultraviolet (UV) flash to the preparation. The light distribution in the focal region and the relative changes in flash intensity with increasing numbers of flashes were quantified with an instrument that integrates the photo-current of a UV-sensitive silicon diode. The function of the set-up was demonstrated by measuring the efficiency of Ca2+ release from DM-nitrophen in quartz capillaries using the Ca2+- sensitive dye antipyrylazo III and by recording the flash-induced recovery of L-type calcium currents in muscle fibres blocked by the light-sensitive dihydropyridine drug nifedipine.

Original languageEnglish
Pages (from-to)385-393
Number of pages9
JournalPflugers Archiv European Journal of Physiology
Volume439
Issue number3
DOIs
Publication statusPublished - 2000

Fingerprint

Photolysis
Petrolatum
Muscle
Light
Muscles
Pilot lights
Fibers
Electric potential
Calcium
Photometers
Xenon
Quartz
Experiments
Accessories
Silicon
Nifedipine
Electric lamps
Semiconductor Lasers
Semiconductor lasers
Skeletal Muscle Fibers

Keywords

  • Caged compounds
  • DM-nitrophen
  • Flash photolysis
  • L-type calcium channel
  • Nifedipine
  • Skeletal muscle
  • Voltage clamp

ASJC Scopus subject areas

  • Physiology

Cite this

Tools for flash-photolysis experiments on voltage-clamped muscle fibre segments. / Schuhmeier, R. P.; Tewes, S.; Szentesi, P.; Melzer, W.

In: Pflugers Archiv European Journal of Physiology, Vol. 439, No. 3, 2000, p. 385-393.

Research output: Contribution to journalArticle

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