Tocopherol Cyclases-Substrate Specificity and Phylogenetic Relations

Jolanta Dłużewska, Renata Szymańska, Michal Gabruk, P. Kós, Beatrycze Nowicka, Jerzy Kruk

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In the present studies, we focused on substrate specificity of tocopherol cyclase, the key enzyme in the biosynthesis of the tocopherols and plastochromanol-8, the main plant lipid antioxidants, with special emphasis on the preference for tocopherols and plastochromanol-8 precursors, taking advantage of the recombinant enzyme originating from Arabidopsis thaliana and isolated plastoglobules, thylakoids and various model systems like micelles and thylakoids. Plastoglobules and triacylglycerol micelles were the most efficient reaction environment for the cyclase. In various investigated systems, synthesis of γ-tocopherol proceeded considerably faster than that of plastochromanol-8, probably mainly due to different localization of the corresponding substrates in the analyzed lipid structures. Moreover, our study was complemented by bioinformatics analysis of the phylogenetic relations of the cyclases and sequence motifs, crucial for the enzyme activity, were proposed. The analysis revealed also a group of tocopherol cyclase-like proteins in a number of heterotrophic bacterial species, with a conserved region common with photosynthetic organisms, that might be engaged in the catalytic activity of both groups of organisms.

Original languageEnglish
Pages (from-to)e0159629
JournalPLoS One
Volume11
Issue number7
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

gamma-tocotrienol
Tocopherols
substrate specificity
Substrate Specificity
tocopherols
Thylakoids
Micelles
micelles
thylakoids
phylogeny
Substrates
Enzymes
Lipids
Biosynthesis
Enzyme activity
autotrophs
Bioinformatics
lipids
enzymes
Computational Biology

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Dłużewska, J., Szymańska, R., Gabruk, M., Kós, P., Nowicka, B., & Kruk, J. (2016). Tocopherol Cyclases-Substrate Specificity and Phylogenetic Relations. PLoS One, 11(7), e0159629. https://doi.org/10.1371/journal.pone.0159629

Tocopherol Cyclases-Substrate Specificity and Phylogenetic Relations. / Dłużewska, Jolanta; Szymańska, Renata; Gabruk, Michal; Kós, P.; Nowicka, Beatrycze; Kruk, Jerzy.

In: PLoS One, Vol. 11, No. 7, 01.01.2016, p. e0159629.

Research output: Contribution to journalArticle

Dłużewska, J, Szymańska, R, Gabruk, M, Kós, P, Nowicka, B & Kruk, J 2016, 'Tocopherol Cyclases-Substrate Specificity and Phylogenetic Relations', PLoS One, vol. 11, no. 7, pp. e0159629. https://doi.org/10.1371/journal.pone.0159629
Dłużewska J, Szymańska R, Gabruk M, Kós P, Nowicka B, Kruk J. Tocopherol Cyclases-Substrate Specificity and Phylogenetic Relations. PLoS One. 2016 Jan 1;11(7):e0159629. https://doi.org/10.1371/journal.pone.0159629
Dłużewska, Jolanta ; Szymańska, Renata ; Gabruk, Michal ; Kós, P. ; Nowicka, Beatrycze ; Kruk, Jerzy. / Tocopherol Cyclases-Substrate Specificity and Phylogenetic Relations. In: PLoS One. 2016 ; Vol. 11, No. 7. pp. e0159629.
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