TNF inhibits myogenesis and downregulates the expression of myogenic regulatory factors myoD and myogenin

Katalin Szalay, Zsolt Rázga, E. Duda

Research output: Contribution to journalArticle

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Abstract

The presence of TNF and other inflammatory cytokines and their receptors is detected during embryonic development, but our knowledge about the role of these proteins in differentiation and development is very limited. TNF modulates the synthesis and activity of a number of transcriptional proteins that regulate the activity of tissue specific genes, therefore it may play a role in normal development. Since its synthesis is upregulated by stress and infections, it may also participate in the induction of pathological developmental processes and malformation. We investigated the effect of TNF in an in vitro differentiation system using C2 myoblasts. This inflammatory cytokine exerted a positive effect on the early steps of the process: it enhanced the proliferation and aggregation of myoblast cells. In contrast, TNF strongly inhibited the expression of those myogenic transcription factors (myoD and myogenin), which are known to be responsible for upregulated activity of muscle specific genes (like the genes of the myofilament proteins), and blocked the synthesis of mRNAs of myogenic differentiation markers (like skeletal α-actin, myosin heavy and light chains). As a result, these cells did not synthesize myofilament proteins and the organization of myofilaments did not take place in TNF-treated myoblasts.

Original languageEnglish
Pages (from-to)391-398
Number of pages8
JournalEuropean Journal of Cell Biology
Volume74
Issue number4
Publication statusPublished - 1997

Fingerprint

Myogenic Regulatory Factors
Myogenin
Muscle Development
Myofibrils
Myoblasts
Down-Regulation
Proteins
Cell Aggregation
Myosin Light Chains
Cytokine Receptors
Myosin Heavy Chains
Differentiation Antigens
Pathologic Processes
Genes
Embryonic Development
Actins
Transcription Factors
Cytokines
Muscles
Messenger RNA

Keywords

  • Myoblast
  • myoD
  • Myogenic differentiation
  • Myogenin
  • Tumor necrosis factor (TNF)

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

TNF inhibits myogenesis and downregulates the expression of myogenic regulatory factors myoD and myogenin. / Szalay, Katalin; Rázga, Zsolt; Duda, E.

In: European Journal of Cell Biology, Vol. 74, No. 4, 1997, p. 391-398.

Research output: Contribution to journalArticle

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