TLR9-mediated signals rescue B-cells from Fas-induced apoptosis via inactivation of caspases

Anikó Hancz, G. Koncz, Dániel Szili, G. Sármay

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The death receptor, CD95/Fas, serves to eliminate potentially dangerous, self-reactive B cells. Engagement of B-cell receptors (BCR) on mature B-cells mediates the escape from cell death resulting in the activation and expansion of antigen specific clones. In addition to the antigen receptors, the receptors of B-cell activating factor belong to the tumor necrosis factor (TNF) family (BAFFR); moreover, the pattern recognition receptor, TLR9 may also deliver survival signals inhibiting Fas-mediated death of B-cells. Our aim was to compare the mechanism of BCR-induced and the BAFFR- or TLR9-stimulated rescue of B-cells from CD95/Fas-mediated apoptosis. We have found that BAFFR and TLR9 collaborate with BCR to protect B-cells from Fas-induced elimination and the rescue is independent of protein synthesis. The results revealed that the TLR9- and BCR-triggered rescue signals are transmitted through partially overlapping pathways; the protein kinase C (PKC) and the abl kinase induced phosphorylation may inactivate caspases in both CpG and anti-IgG stimulated cells. However, PI3-K activation is crucial upon the BCR driven anti-apoptotic effect, while p38 MAPK-mediated inactivation of caspases seems to play essential role in TLR9-mediated protection against Fas-induced programmed cell death.

Original languageEnglish
Pages (from-to)77-84
Number of pages8
JournalImmunology Letters
Volume143
Issue number1
DOIs
Publication statusPublished - Mar 30 2012

Fingerprint

Caspases
B-Lymphocytes
Apoptosis
Cell Death
Interleukin-4 Receptors
Pattern Recognition Receptors
Death Domain Receptors
Antigen Receptors
p38 Mitogen-Activated Protein Kinases
Clone Cells
Tumor Necrosis Factor-alpha
Phosphorylation
Antigens

Keywords

  • Apoptosis
  • B-cells
  • BAFF
  • Fas
  • TLR9

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

TLR9-mediated signals rescue B-cells from Fas-induced apoptosis via inactivation of caspases. / Hancz, Anikó; Koncz, G.; Szili, Dániel; Sármay, G.

In: Immunology Letters, Vol. 143, No. 1, 30.03.2012, p. 77-84.

Research output: Contribution to journalArticle

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