TLC-DAR for the analysis of biological samples. A newly developed rapid tool for studying drug metabolism

Imre Klebovich, József Szúnyog, István Hazai

Research output: Contribution to journalShort survey

18 Citations (Scopus)

Abstract

This report, illustrated with practical examples, summarizes the advantages and disadvantages of combined thin-layer chromatography-digital autoradiography (TLC-DAR) and other techniques (hereafter denoted 'non-TLC-DAR techniques') used for plate evaluation in planar chromatography. It is demonstrated that this newly developed method can he widely used for the analysis of a variety of biological samples and, because information is obtained quickly at low cost, it is an excellent tool in metabolism research. DAR is a convenient and extremely rapid means of locating and quantifying radioactive metabolites on the TLC plate after separation and/or purification. TLC-DAR can be successfully used to monitor intermediates and/or end products in the synthesis of radiolabeled compounds, to determine the radiochemical purity of chemicals, and to investigate the radiochemical stability of compounds in studies of the metabolism of xenobiotics. Using deramciclane (a new anxiolytic compound) as an example, it has been shown that DAR can be successfully used to obtain information rapidly in different phases of the study of in-vitro and in-vivo metabolism This report summarizes the applications and limitations of TLC-DAR in drug metabolism research and compares various techniques used for detection of beta-emitting isotopes.

Original languageEnglish
Pages (from-to)399-405
Number of pages7
JournalJournal of Planar Chromatography - Modern TLC
Volume10
Issue number6
Publication statusPublished - Nov 1 1997

Keywords

  • Deramciclane (EGIS-3886)
  • Digital autoradiography (DAR)
  • Imaging techniques
  • Metabolism
  • TLC-DAR

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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