TLC analysis to determine the seasonal dependence of the level of 20-hydroxyecdysone in the leaves of Serratula tinctoria (L.)

Mária Báthori, Imre Máthé, Lajos Praszna, Katalin Rischák, Huba Kalász

Research output: Contribution to journalArticle

11 Citations (Scopus)


A quantitative method has been developed for determination of the 20-hydroxyecdysone content of Serratula tinctoria (L.). Thin-layer chromatography on silica was performed in two consecutive steps; the first was with chloroform - ethanol and the separation was completed with chloroform - methanol - benzene. Plant samples of different phenophases were collected in 1994, ecdysteroids were extracted, and, after a clean-up procedure, the 20-hydroxyecdysone content was determined by TLC - densitometry.

Original languageEnglish
Pages (from-to)264-268
Number of pages5
JournalJournal of Planar Chromatography - Modern TLC
Issue number4
Publication statusPublished - Oct 4 1996



  • Densitometry
  • Quantitative TLC
  • Seasonal dependence of 20-hydroxyecdysone content

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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