Tissue tropism of highly pathogenic avian influenza virus subtype h5n1 in naturally infected mute swans (cygnus olor), domestic geese (anser anser var. domestica), pekin ducks (anas platyrhynchos) and mulard ducks (cairina moschata × anas platyrhynchos)

L. Szeredi, A. Dán, Nimród Pálmai, K. Ursu, A. Bálint, Zsófia Szeleczky, E. Ivánics, K. Erdélyi, Dóra Rigó, Lajos Tekes, Róbert Glávits

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Abstract

The 2006 epidemic due to highly pathogenic avian influenza virus (HPAIV) subtype H5N1 in Hungary caused the most severe losses in waterfowl which were, according to the literature at the time, supposed to be the most resistant to this pathogen. The presence of pathological lesions and the amount of viral antigen were quantified by gross pathology, histopathology and immunohistochemistry (IHC) in the organs of four waterfowl species [mute swans (n = 10), domestic geese (n = 6), mulard ducks (n = 6) and Pekin ducks (n = 5)] collected during the epidemic. H5N1 subtype HPAIV was isolated from all birds examined. Quantitative real-time reverse transcriptase-polymerase chain reaction (qRRT-PCR) was also applied on a subset of samples [domestic geese (n = 3), mulard (n = 4) and Pekin duck (n = 4)] in order to compare its sensitivity with IHC. Viral antigen was detected by IHC in all cases. However, the overall presence of viral antigen in tissue samples was quite variable: virus antigen was present in 56/81 (69%) swan, 22/38 (58%) goose, 28/46 (61%) mulard duck and 5/43 (12%) Pekin duck tissue samples. HPAIV subtype H5N1 was detected by qRRT-PCR in all birds examined, in 19/19 (100%) goose, 7/28 (25%) mulard duck and 12/28 (43%) Pekin duck tissue samples. As compared to qRRTPCR, the IHC was less sensitive in geese and Pekin ducks but more sensitive in mulard ducks. The IHC was consistently positive above 4.31 log10 copies/reaction but it gave very variable results below that level. Neurotropism of the isolated virus strains was demonstrated by finding the largest amount of viral antigen and the highest average RNA load in the brain in all four waterfowl species examined.

Original languageEnglish
Pages (from-to)133-145
Number of pages13
JournalActa Veterinaria Hungarica
Volume58
Issue number1
DOIs
Publication statusPublished - Mar 1 2010

Fingerprint

Cygnus olor
tissue tropism
Cairina moschata
Anser anser
Geese
Pekin
Tropism
Influenza in Birds
Ducks
Anas platyrhynchos
geese
Orthomyxoviridae
Influenza A virus
ducks
Viral Antigens
viral antigens
immunohistochemistry
Immunohistochemistry
waterfowl
Reverse Transcriptase Polymerase Chain Reaction

Keywords

  • H5N1
  • Highly pathogenic avian influenza virus
  • Natural infection
  • Polymerase chain reaction
  • Viral antigen
  • Waterfowl

ASJC Scopus subject areas

  • veterinary(all)

Cite this

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title = "Tissue tropism of highly pathogenic avian influenza virus subtype h5n1 in naturally infected mute swans (cygnus olor), domestic geese (anser anser var. domestica), pekin ducks (anas platyrhynchos) and mulard ducks (cairina moschata × anas platyrhynchos)",
abstract = "The 2006 epidemic due to highly pathogenic avian influenza virus (HPAIV) subtype H5N1 in Hungary caused the most severe losses in waterfowl which were, according to the literature at the time, supposed to be the most resistant to this pathogen. The presence of pathological lesions and the amount of viral antigen were quantified by gross pathology, histopathology and immunohistochemistry (IHC) in the organs of four waterfowl species [mute swans (n = 10), domestic geese (n = 6), mulard ducks (n = 6) and Pekin ducks (n = 5)] collected during the epidemic. H5N1 subtype HPAIV was isolated from all birds examined. Quantitative real-time reverse transcriptase-polymerase chain reaction (qRRT-PCR) was also applied on a subset of samples [domestic geese (n = 3), mulard (n = 4) and Pekin duck (n = 4)] in order to compare its sensitivity with IHC. Viral antigen was detected by IHC in all cases. However, the overall presence of viral antigen in tissue samples was quite variable: virus antigen was present in 56/81 (69{\%}) swan, 22/38 (58{\%}) goose, 28/46 (61{\%}) mulard duck and 5/43 (12{\%}) Pekin duck tissue samples. HPAIV subtype H5N1 was detected by qRRT-PCR in all birds examined, in 19/19 (100{\%}) goose, 7/28 (25{\%}) mulard duck and 12/28 (43{\%}) Pekin duck tissue samples. As compared to qRRTPCR, the IHC was less sensitive in geese and Pekin ducks but more sensitive in mulard ducks. The IHC was consistently positive above 4.31 log10 copies/reaction but it gave very variable results below that level. Neurotropism of the isolated virus strains was demonstrated by finding the largest amount of viral antigen and the highest average RNA load in the brain in all four waterfowl species examined.",
keywords = "H5N1, Highly pathogenic avian influenza virus, Natural infection, Polymerase chain reaction, Viral antigen, Waterfowl",
author = "L. Szeredi and A. D{\'a}n and Nimr{\'o}d P{\'a}lmai and K. Ursu and A. B{\'a}lint and Zs{\'o}fia Szeleczky and E. Iv{\'a}nics and K. Erd{\'e}lyi and D{\'o}ra Rig{\'o} and Lajos Tekes and R{\'o}bert Gl{\'a}vits",
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T1 - Tissue tropism of highly pathogenic avian influenza virus subtype h5n1 in naturally infected mute swans (cygnus olor), domestic geese (anser anser var. domestica), pekin ducks (anas platyrhynchos) and mulard ducks (cairina moschata × anas platyrhynchos)

AU - Szeredi, L.

AU - Dán, A.

AU - Pálmai, Nimród

AU - Ursu, K.

AU - Bálint, A.

AU - Szeleczky, Zsófia

AU - Ivánics, E.

AU - Erdélyi, K.

AU - Rigó, Dóra

AU - Tekes, Lajos

AU - Glávits, Róbert

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N2 - The 2006 epidemic due to highly pathogenic avian influenza virus (HPAIV) subtype H5N1 in Hungary caused the most severe losses in waterfowl which were, according to the literature at the time, supposed to be the most resistant to this pathogen. The presence of pathological lesions and the amount of viral antigen were quantified by gross pathology, histopathology and immunohistochemistry (IHC) in the organs of four waterfowl species [mute swans (n = 10), domestic geese (n = 6), mulard ducks (n = 6) and Pekin ducks (n = 5)] collected during the epidemic. H5N1 subtype HPAIV was isolated from all birds examined. Quantitative real-time reverse transcriptase-polymerase chain reaction (qRRT-PCR) was also applied on a subset of samples [domestic geese (n = 3), mulard (n = 4) and Pekin duck (n = 4)] in order to compare its sensitivity with IHC. Viral antigen was detected by IHC in all cases. However, the overall presence of viral antigen in tissue samples was quite variable: virus antigen was present in 56/81 (69%) swan, 22/38 (58%) goose, 28/46 (61%) mulard duck and 5/43 (12%) Pekin duck tissue samples. HPAIV subtype H5N1 was detected by qRRT-PCR in all birds examined, in 19/19 (100%) goose, 7/28 (25%) mulard duck and 12/28 (43%) Pekin duck tissue samples. As compared to qRRTPCR, the IHC was less sensitive in geese and Pekin ducks but more sensitive in mulard ducks. The IHC was consistently positive above 4.31 log10 copies/reaction but it gave very variable results below that level. Neurotropism of the isolated virus strains was demonstrated by finding the largest amount of viral antigen and the highest average RNA load in the brain in all four waterfowl species examined.

AB - The 2006 epidemic due to highly pathogenic avian influenza virus (HPAIV) subtype H5N1 in Hungary caused the most severe losses in waterfowl which were, according to the literature at the time, supposed to be the most resistant to this pathogen. The presence of pathological lesions and the amount of viral antigen were quantified by gross pathology, histopathology and immunohistochemistry (IHC) in the organs of four waterfowl species [mute swans (n = 10), domestic geese (n = 6), mulard ducks (n = 6) and Pekin ducks (n = 5)] collected during the epidemic. H5N1 subtype HPAIV was isolated from all birds examined. Quantitative real-time reverse transcriptase-polymerase chain reaction (qRRT-PCR) was also applied on a subset of samples [domestic geese (n = 3), mulard (n = 4) and Pekin duck (n = 4)] in order to compare its sensitivity with IHC. Viral antigen was detected by IHC in all cases. However, the overall presence of viral antigen in tissue samples was quite variable: virus antigen was present in 56/81 (69%) swan, 22/38 (58%) goose, 28/46 (61%) mulard duck and 5/43 (12%) Pekin duck tissue samples. HPAIV subtype H5N1 was detected by qRRT-PCR in all birds examined, in 19/19 (100%) goose, 7/28 (25%) mulard duck and 12/28 (43%) Pekin duck tissue samples. As compared to qRRTPCR, the IHC was less sensitive in geese and Pekin ducks but more sensitive in mulard ducks. The IHC was consistently positive above 4.31 log10 copies/reaction but it gave very variable results below that level. Neurotropism of the isolated virus strains was demonstrated by finding the largest amount of viral antigen and the highest average RNA load in the brain in all four waterfowl species examined.

KW - H5N1

KW - Highly pathogenic avian influenza virus

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KW - Polymerase chain reaction

KW - Viral antigen

KW - Waterfowl

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