"Tissue" transglutaminase is specifically expressed in neonatal rat liver cells undergoing apoptosis upon epidermal growth factor-stimulation

M. Piacentini, F. Autuori, L. Dini, M. G. Farrace, L. Ghibelli, L. Piredda, L. Fésüs

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

We recently reported that activation of "tissue" transglutaminase (EC 2.3.2.13; tTG) in liver cells undergoing apoptosis determines extensive cross-linking of cellular proteins resulting in the formation of SDS-insoluble shells in the so-called "apoptotic bodies". In attempt to obtain further insight into the role played by tTG in apoptosis of liver cells, we investigated its expression in primary cultures of neonatal rat liver cells stimulated with epidermal growth factor (EGF). EGF-treatment of neonatal rat liver cells induces first hyperplasia of hepatocytes, followed by involution characterized by a high incidence of apoptosis. The proliferative phase of hepatocytes is paralleled by a 10-fold increase in tTG mRNA level, which is followed, during the phase of involution, by sequential increases in enzyme activity and levels of SDS-insoluble apoptotic bodies. tTG immunostaining at both the light- and electron-microscopic levels shows that the most intensive reaction is present in globular structures showing the typical morphological appearance of mature apoptotic bodies. In early apoptotic stages, tTG protein is localized in the perinuclear region of the cell. Intense immunostaining is also found in the apoptotic bodies present inside phagosomes within the cytoplasm of neighboring cells. This evidence confirms and extends our previous findings, indicating that tTG induction and activation specifically takes place in cells undergoing apoptosis, suggesting a key role for the enzyme in the apoptotic program.

Original languageEnglish
Pages (from-to)227-235
Number of pages9
JournalCell and Tissue Research
Volume263
Issue number2
DOIs
Publication statusPublished - Feb 1991

Fingerprint

Epidermal Growth Factor
Liver
Rats
Apoptosis
Chemical activation
Enzyme activity
Hepatocytes
Proteins
Phagosomes
Messenger RNA
Enzymes
transglutaminase 2
Electrons
Hyperplasia
Cytoplasm
Light
Extracellular Vesicles
Incidence

Keywords

  • Apoptosis
  • Cell culture
  • Cell proliferation
  • Epidermal growth factor (EGF)
  • Neonatal hepatocytes

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

"Tissue" transglutaminase is specifically expressed in neonatal rat liver cells undergoing apoptosis upon epidermal growth factor-stimulation. / Piacentini, M.; Autuori, F.; Dini, L.; Farrace, M. G.; Ghibelli, L.; Piredda, L.; Fésüs, L.

In: Cell and Tissue Research, Vol. 263, No. 2, 02.1991, p. 227-235.

Research output: Contribution to journalArticle

Piacentini, M. ; Autuori, F. ; Dini, L. ; Farrace, M. G. ; Ghibelli, L. ; Piredda, L. ; Fésüs, L. / "Tissue" transglutaminase is specifically expressed in neonatal rat liver cells undergoing apoptosis upon epidermal growth factor-stimulation. In: Cell and Tissue Research. 1991 ; Vol. 263, No. 2. pp. 227-235.
@article{c2c667bf156c4e15a0646f32cf8601ad,
title = "{"}Tissue{"} transglutaminase is specifically expressed in neonatal rat liver cells undergoing apoptosis upon epidermal growth factor-stimulation",
abstract = "We recently reported that activation of {"}tissue{"} transglutaminase (EC 2.3.2.13; tTG) in liver cells undergoing apoptosis determines extensive cross-linking of cellular proteins resulting in the formation of SDS-insoluble shells in the so-called {"}apoptotic bodies{"}. In attempt to obtain further insight into the role played by tTG in apoptosis of liver cells, we investigated its expression in primary cultures of neonatal rat liver cells stimulated with epidermal growth factor (EGF). EGF-treatment of neonatal rat liver cells induces first hyperplasia of hepatocytes, followed by involution characterized by a high incidence of apoptosis. The proliferative phase of hepatocytes is paralleled by a 10-fold increase in tTG mRNA level, which is followed, during the phase of involution, by sequential increases in enzyme activity and levels of SDS-insoluble apoptotic bodies. tTG immunostaining at both the light- and electron-microscopic levels shows that the most intensive reaction is present in globular structures showing the typical morphological appearance of mature apoptotic bodies. In early apoptotic stages, tTG protein is localized in the perinuclear region of the cell. Intense immunostaining is also found in the apoptotic bodies present inside phagosomes within the cytoplasm of neighboring cells. This evidence confirms and extends our previous findings, indicating that tTG induction and activation specifically takes place in cells undergoing apoptosis, suggesting a key role for the enzyme in the apoptotic program.",
keywords = "Apoptosis, Cell culture, Cell proliferation, Epidermal growth factor (EGF), Neonatal hepatocytes",
author = "M. Piacentini and F. Autuori and L. Dini and Farrace, {M. G.} and L. Ghibelli and L. Piredda and L. F{\'e}s{\"u}s",
year = "1991",
month = "2",
doi = "10.1007/BF00318764",
language = "English",
volume = "263",
pages = "227--235",
journal = "Cell and Tissue Research",
issn = "0302-766X",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - "Tissue" transglutaminase is specifically expressed in neonatal rat liver cells undergoing apoptosis upon epidermal growth factor-stimulation

AU - Piacentini, M.

AU - Autuori, F.

AU - Dini, L.

AU - Farrace, M. G.

AU - Ghibelli, L.

AU - Piredda, L.

AU - Fésüs, L.

PY - 1991/2

Y1 - 1991/2

N2 - We recently reported that activation of "tissue" transglutaminase (EC 2.3.2.13; tTG) in liver cells undergoing apoptosis determines extensive cross-linking of cellular proteins resulting in the formation of SDS-insoluble shells in the so-called "apoptotic bodies". In attempt to obtain further insight into the role played by tTG in apoptosis of liver cells, we investigated its expression in primary cultures of neonatal rat liver cells stimulated with epidermal growth factor (EGF). EGF-treatment of neonatal rat liver cells induces first hyperplasia of hepatocytes, followed by involution characterized by a high incidence of apoptosis. The proliferative phase of hepatocytes is paralleled by a 10-fold increase in tTG mRNA level, which is followed, during the phase of involution, by sequential increases in enzyme activity and levels of SDS-insoluble apoptotic bodies. tTG immunostaining at both the light- and electron-microscopic levels shows that the most intensive reaction is present in globular structures showing the typical morphological appearance of mature apoptotic bodies. In early apoptotic stages, tTG protein is localized in the perinuclear region of the cell. Intense immunostaining is also found in the apoptotic bodies present inside phagosomes within the cytoplasm of neighboring cells. This evidence confirms and extends our previous findings, indicating that tTG induction and activation specifically takes place in cells undergoing apoptosis, suggesting a key role for the enzyme in the apoptotic program.

AB - We recently reported that activation of "tissue" transglutaminase (EC 2.3.2.13; tTG) in liver cells undergoing apoptosis determines extensive cross-linking of cellular proteins resulting in the formation of SDS-insoluble shells in the so-called "apoptotic bodies". In attempt to obtain further insight into the role played by tTG in apoptosis of liver cells, we investigated its expression in primary cultures of neonatal rat liver cells stimulated with epidermal growth factor (EGF). EGF-treatment of neonatal rat liver cells induces first hyperplasia of hepatocytes, followed by involution characterized by a high incidence of apoptosis. The proliferative phase of hepatocytes is paralleled by a 10-fold increase in tTG mRNA level, which is followed, during the phase of involution, by sequential increases in enzyme activity and levels of SDS-insoluble apoptotic bodies. tTG immunostaining at both the light- and electron-microscopic levels shows that the most intensive reaction is present in globular structures showing the typical morphological appearance of mature apoptotic bodies. In early apoptotic stages, tTG protein is localized in the perinuclear region of the cell. Intense immunostaining is also found in the apoptotic bodies present inside phagosomes within the cytoplasm of neighboring cells. This evidence confirms and extends our previous findings, indicating that tTG induction and activation specifically takes place in cells undergoing apoptosis, suggesting a key role for the enzyme in the apoptotic program.

KW - Apoptosis

KW - Cell culture

KW - Cell proliferation

KW - Epidermal growth factor (EGF)

KW - Neonatal hepatocytes

UR - http://www.scopus.com/inward/record.url?scp=0026018673&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026018673&partnerID=8YFLogxK

U2 - 10.1007/BF00318764

DO - 10.1007/BF00318764

M3 - Article

C2 - 1672508

AN - SCOPUS:0026018673

VL - 263

SP - 227

EP - 235

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

IS - 2

ER -