Tissue transglutaminase-dependent posttranslational modification of the retinoblastoma gene product in promonocytic cells undergoing apoptosis

Serafina Oliverio, Alessandra Amendola, Federica Di Sano, Maria Grazia Farrace, L. Fésüs, Zoltan Nemes, Lucia Piredda, Angelo Spinedi, Mauro Piacentini

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Abstract

The retinoblastoma gene product (pRB) plays an important role in controlling both cell release from the G1 phase and apoptosis. We show here that in the early phases of apoptosis, pRB is posttranslationally modified by a tissue transglutaminase (tTG)-catalyzed reaction. In fact, by employing a novel haptenized lysis synthetic substrate which allows the isolation of glutaminyl-tTG substrates in vivo, we identified pRB as a potential tTG substrate in U937 cells undergoing apoptosis. In keeping with this finding, we showed that apoptosis of U937 cells is characterized by the rapid disappearance of the 105,000- to 110,000-molecular-weight pRB forms concomitantly with the appearance of a smear of immunoreactive products with a molecular weight of greater than 250,000. The shift in pRB molecular weight was reproduced by adding exogenous purified tTG to extracts obtained from viable U937 cells and was prevented by dansylcadaverine, a potent enzyme inhibitor. The effect of the pRB posttranslational modification during apoptosis was investigated by determining the E2F-1 levels and by isolating and characterizing pRB-null clones from U937 cells. Notably, the lack of pRB in these U937-derived clones renders these p53-null cells highly resistant to apoptosis induced by serum withdrawal, calphostin C, and ceramide. Taken together, these data suggest that tTG, acting on the pRB protein, might play an important role in the cell progression through the death program.

Original languageEnglish
Pages (from-to)6040-6048
Number of pages9
JournalMolecular and Cellular Biology
Volume17
Issue number10
Publication statusPublished - Oct 1997

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Retinoblastoma Genes
Post Translational Protein Processing
U937 Cells
Apoptosis
Null Lymphocytes
Molecular Weight
Clone Cells
Ceramides
G1 Phase
Enzyme Inhibitors
transglutaminase 2
Serum

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Tissue transglutaminase-dependent posttranslational modification of the retinoblastoma gene product in promonocytic cells undergoing apoptosis. / Oliverio, Serafina; Amendola, Alessandra; Di Sano, Federica; Farrace, Maria Grazia; Fésüs, L.; Nemes, Zoltan; Piredda, Lucia; Spinedi, Angelo; Piacentini, Mauro.

In: Molecular and Cellular Biology, Vol. 17, No. 10, 10.1997, p. 6040-6048.

Research output: Contribution to journalArticle

Oliverio, S, Amendola, A, Di Sano, F, Farrace, MG, Fésüs, L, Nemes, Z, Piredda, L, Spinedi, A & Piacentini, M 1997, 'Tissue transglutaminase-dependent posttranslational modification of the retinoblastoma gene product in promonocytic cells undergoing apoptosis', Molecular and Cellular Biology, vol. 17, no. 10, pp. 6040-6048.
Oliverio, Serafina ; Amendola, Alessandra ; Di Sano, Federica ; Farrace, Maria Grazia ; Fésüs, L. ; Nemes, Zoltan ; Piredda, Lucia ; Spinedi, Angelo ; Piacentini, Mauro. / Tissue transglutaminase-dependent posttranslational modification of the retinoblastoma gene product in promonocytic cells undergoing apoptosis. In: Molecular and Cellular Biology. 1997 ; Vol. 17, No. 10. pp. 6040-6048.
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