Tissue-specific signal(s) activate the promoter of a metallocarboxypeptidase inhibitor gene family in potato tuber and berry

Attila Molnár, Ágnes Lovas, Z. Bánfalvi, L. Lakatos, Zsolt Polgár, Sándor Horváth

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The molecular basis of the differential expression of the GM7-type metallocarboxypeptidase inhibitor (MCPI) genes in tuberizing (StMCPI) and non-tuberizing Solanum species (SbMCPI) was investigated. It was shown that the StMCPI is encoded by a gene family in Solanum tuberosum (potato), but SbMCPI might be a single-copy gene in the non-tuberizing species Solanum brevidens. The StMCPI promoter shows evolutionary relatedness to the S. brevidens-derived SbMCPI and to the fruit-specific tomato promoter 2A11. Both StMCPI and SbMCPI promoter regions were able to confer tuber- and berry-specific expression for the β-glucuronidase reporter gene in potato suggesting that the difference in MCPI gene expression is in trans regulatory factors between the tuberizing and the non-tuberizing Solanum species. The MCPI promoters did not respond to metabolic, environmental or hormonal signals in leaves. Thus, the MCPI genes are regulated in a different way than the other known tuber-specific genes and potentially are suitable for biotechnological application in potato to provide specific transgene expression in tuber and berry.

Original languageEnglish
Pages (from-to)301-311
Number of pages11
JournalPlant Molecular Biology
Volume46
Issue number3
DOIs
Publication statusPublished - 2001

Fingerprint

metallocarboxypeptidases
Solanum tuberosum
small fruits
Fruit
tubers
Solanum
Genes
promoter regions
potatoes
Tissue
Solanum palustre
genes
Glucuronidase
Lycopersicon esculentum
Transgenes
Reporter Genes
Genetic Promoter Regions
reporter genes
transgenes
Fruits

Keywords

  • Berry
  • Metallocarboxypeptidase inhibitor
  • Organ-specific gene expression
  • Potato
  • Tomato
  • Tuber

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Tissue-specific signal(s) activate the promoter of a metallocarboxypeptidase inhibitor gene family in potato tuber and berry. / Molnár, Attila; Lovas, Ágnes; Bánfalvi, Z.; Lakatos, L.; Polgár, Zsolt; Horváth, Sándor.

In: Plant Molecular Biology, Vol. 46, No. 3, 2001, p. 301-311.

Research output: Contribution to journalArticle

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