Tissue specific protochlorophyll(ide) forms in dark-forced shoots of grapevine (Vitis vinifera L.)

B. Böddi, K. Bóka, C. Sundqvist

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cuttings of grapevine (Vitis vinifera L. cv. Chardonnay) were dark-forced at least three weeks. Pigment contents, 77 K fluorescence emission, excitation spectra of the leaves, petioles, stems, transmission electron micrographs of the etioplasts from leaves, the chlorenchyma tissues of the stems were analysed. The dark-grown leaves, stems contained 8 to 10, 3 to 5 μg/g fresh weight protochlorophyllide, its esters, respectively. HPLC analysis showed that the molar ratio of the unesterified, esterified pigments was 7:3 in the shoot developed in darkness. The dark-forced leaves contained carotenoids identified as: neoxanthin, violaxanthin, antheraxanthin, lutein, β-carotene. Detailed analyses of the fluorescence spectra proved that all tissues of the dark-forced shoots had protochlorophyllide or protochlorophyll forms with emission maxima at 628, 636, 644, 655, 669 nm. The 628, 636 nm emitting forms were present in all parts of the dark-forced shoot, but dominated in the stems, which may indicate an organ specificity of the etioplast development. Variations in the distribution of the pigment forms were even found in the different tissues of the stem. The subepidermal layers were more abundant in the 655 nm form than the parenchyma cells of the inner part of the cortex, the pith. In the latter cells, the plastid differentiation stopped in intermediary stages between proplastids, etioplasts. The plastids in the subepidermal layers had developed prolamellar body structures, which were similar to those of etiolated leaves. The results highlight the importance of organ-, tissue specificity of plastid differentiation for chlorophyll biosynthesis, greening of different plant organs.

Original languageEnglish
Pages (from-to)141-150
Number of pages10
JournalPhotosynthesis Research
Volume82
Issue number2
DOIs
Publication statusPublished - 2004

Fingerprint

protochlorophyll
Organ Specificity
Plastids
Vitis
Protochlorophyllide
Chloroplasts
Vitis vinifera
etioplasts
Pigments
Carotenoids
Tissue
stems
shoots
plastids
protochlorophyllides
Fluorescence
pigments
leaves
Lutein
Darkness

Keywords

  • Chlorenchyma
  • Dark-forcing
  • Etioplast
  • Fluorescence
  • Grapevine
  • Plastid
  • Protochlorophyllide

ASJC Scopus subject areas

  • Plant Science

Cite this

Tissue specific protochlorophyll(ide) forms in dark-forced shoots of grapevine (Vitis vinifera L.). / Böddi, B.; Bóka, K.; Sundqvist, C.

In: Photosynthesis Research, Vol. 82, No. 2, 2004, p. 141-150.

Research output: Contribution to journalArticle

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AB - Cuttings of grapevine (Vitis vinifera L. cv. Chardonnay) were dark-forced at least three weeks. Pigment contents, 77 K fluorescence emission, excitation spectra of the leaves, petioles, stems, transmission electron micrographs of the etioplasts from leaves, the chlorenchyma tissues of the stems were analysed. The dark-grown leaves, stems contained 8 to 10, 3 to 5 μg/g fresh weight protochlorophyllide, its esters, respectively. HPLC analysis showed that the molar ratio of the unesterified, esterified pigments was 7:3 in the shoot developed in darkness. The dark-forced leaves contained carotenoids identified as: neoxanthin, violaxanthin, antheraxanthin, lutein, β-carotene. Detailed analyses of the fluorescence spectra proved that all tissues of the dark-forced shoots had protochlorophyllide or protochlorophyll forms with emission maxima at 628, 636, 644, 655, 669 nm. The 628, 636 nm emitting forms were present in all parts of the dark-forced shoot, but dominated in the stems, which may indicate an organ specificity of the etioplast development. Variations in the distribution of the pigment forms were even found in the different tissues of the stem. The subepidermal layers were more abundant in the 655 nm form than the parenchyma cells of the inner part of the cortex, the pith. In the latter cells, the plastid differentiation stopped in intermediary stages between proplastids, etioplasts. The plastids in the subepidermal layers had developed prolamellar body structures, which were similar to those of etiolated leaves. The results highlight the importance of organ-, tissue specificity of plastid differentiation for chlorophyll biosynthesis, greening of different plant organs.

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