In the present study the authors describe the Tissue Micro-Array (TMA), also known as the tissue microseries technique, a novel histopathological procedure currently applied in the pathological practice. During TMA tissue microseries capable for storing more than a thousand specimens are obtained manually or automatically removing cylindrical tissue sections from classical paraffin-embedded blocks, following deparaffinization. The described method permits considerable savings of reagents and the examination of several specimens on only one slide. The authors also describe the laboratory manual instrument kit developed by Hungarian researchers, suitable for obtaining the tissue microseries, as well as the operations performed by using this kit: the marking of the representative tissue areas on the classical histopathological slides under light microscope, the removal of the areas of interest from the paraffin-embedded blocks, followed by the insertion of these tissue sections in tissue multiblocks presenting a series of holes of identical proportions and the preparation of histopathological sections. The authors also illustrate the procedures described in the present study.
|Translated title of the contribution||Tissue microseries (tissue micro-array - TMA) technique in the veterinary oncopathological practice|
|Number of pages||6|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - Dec 1 2007|
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