Tissue factor is expressed on monocytes during simulated extracorporeal circulation

J. Kappelmayer, A. Bernabei, L. H. Edmunds, T. S. Edgington, R. W. Colman

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Certain forms of extracorporeal circulation exemplified by cardiopulmonary bypass require continuous high-dose anticoagulation to prevent thromboembolic complications. We hypothesized that monocytes may be stimulated to express tissue factor (TF) during prolonged simulated extracorporeal circulation. TF was identified both by flow cytometry by using three TF-specific monoclonal antibodies and functional assay of procoagulant activity (PCA). TF significantly increased between 2 and 6 hours of simulated extracorporeal circulation by both analyses. Relative fluorescence on monocytes increased from a control value of 100 to 313±79 on cells from the simulated extracorporeal circuit (p6 monocytes (p6 monocytes (p

Original languageEnglish
Pages (from-to)1075-1081
Number of pages7
JournalCirculation Research
Volume72
Issue number5
Publication statusPublished - 1993

Fingerprint

Extracorporeal Circulation
Thromboplastin
Monocytes
Cardiopulmonary Bypass
Flow Cytometry
Fluorescence
Monoclonal Antibodies

Keywords

  • cardiopulmonary bypass
  • tissue factor

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Kappelmayer, J., Bernabei, A., Edmunds, L. H., Edgington, T. S., & Colman, R. W. (1993). Tissue factor is expressed on monocytes during simulated extracorporeal circulation. Circulation Research, 72(5), 1075-1081.

Tissue factor is expressed on monocytes during simulated extracorporeal circulation. / Kappelmayer, J.; Bernabei, A.; Edmunds, L. H.; Edgington, T. S.; Colman, R. W.

In: Circulation Research, Vol. 72, No. 5, 1993, p. 1075-1081.

Research output: Contribution to journalArticle

Kappelmayer, J, Bernabei, A, Edmunds, LH, Edgington, TS & Colman, RW 1993, 'Tissue factor is expressed on monocytes during simulated extracorporeal circulation', Circulation Research, vol. 72, no. 5, pp. 1075-1081.
Kappelmayer, J. ; Bernabei, A. ; Edmunds, L. H. ; Edgington, T. S. ; Colman, R. W. / Tissue factor is expressed on monocytes during simulated extracorporeal circulation. In: Circulation Research. 1993 ; Vol. 72, No. 5. pp. 1075-1081.
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