Tissue-associated phenotypic heterogeneity of peripheral B cells in mice

P. Balogh, A. Kumanovics

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

After their primary differentiation and selection in the bone marrow, the cells of B lineage are distributed to the peripheral lymphoid system. Here we report that, with the use of a novel rat monoclonal antibody (IBL-2), a tissue-related phenotypic difference could be observed in the peripheral B-cell compartment in mouse. The antigen recognized by this antibody is a 25,000/29,000 MW heterodimeric cell surface molecule which is resistant to phosphatidylinositolphospholipase C treatment, but is sensitive to proteases. The antigen was found to be expressed by the majority of B cells from the spleen, whereas the B cells from other peripheral sources (lymph nodes and Peyer's patches) proved to be negative. The staining pattern of splenic B cells was heterogeneous, containing a substantial dim population (IBL-2(lo)), and a smaller, intensely stained fraction (IBL-2(hi)) within the positive subset. Unlike the B cells, the T cells were negative in every peripheral lymphoid tissue analysed. In addition, the ratios between the various IBL-2-reactive B cells (positive to negative and, within the positive population, the IBL-2(lo) to IBL-2(hi), respectively) in the spleen were quite similar to that of B cells in the bone marrow. Furthermore, the levels of L-selectin expressed by the various IBL-2-reactive subpopulations were found to be heterogeneous both in the bone marrow and in the spleen. The bone marrow cells could be resolved into double negative, L-selectin(±)/IBL-2(lo), L-selectin--IBL-2(lo), and L-selectin-/IBL-2(hi) populations, respectively. In the spleen, an additional fraction with L-selectin+/IBL-2- phenotype could be detected. In both tissues, the overwhelming majority of IBL-2(hi) cells were found at the MEL-14- compartment. We conclude that either these findings may reflect a heterogeneous development state within the peripheral B-cell pool, with a substantial fraction of splenic B cells being less differentiated than those in other peripheral lymphoid tissues, or alternatively, the differential reactivity of murine B cells with the IBL-2 monoclonal antibody is due to their tissue location.

Original languageEnglish
Pages (from-to)560-567
Number of pages8
JournalImmunology
Volume86
Issue number4
Publication statusPublished - 1995

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B-Lymphocytes
L-Selectin
Spleen
Lymphoid Tissue
Bone Marrow Cells
Bone Marrow
Monoclonal Antibodies
Population
Antigens
Peyer's Patches
Cell Lineage
Peptide Hydrolases
Lymph Nodes
Staining and Labeling
T-Lymphocytes
Phenotype
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Tissue-associated phenotypic heterogeneity of peripheral B cells in mice. / Balogh, P.; Kumanovics, A.

In: Immunology, Vol. 86, No. 4, 1995, p. 560-567.

Research output: Contribution to journalArticle

Balogh, P. ; Kumanovics, A. / Tissue-associated phenotypic heterogeneity of peripheral B cells in mice. In: Immunology. 1995 ; Vol. 86, No. 4. pp. 560-567.
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AB - After their primary differentiation and selection in the bone marrow, the cells of B lineage are distributed to the peripheral lymphoid system. Here we report that, with the use of a novel rat monoclonal antibody (IBL-2), a tissue-related phenotypic difference could be observed in the peripheral B-cell compartment in mouse. The antigen recognized by this antibody is a 25,000/29,000 MW heterodimeric cell surface molecule which is resistant to phosphatidylinositolphospholipase C treatment, but is sensitive to proteases. The antigen was found to be expressed by the majority of B cells from the spleen, whereas the B cells from other peripheral sources (lymph nodes and Peyer's patches) proved to be negative. The staining pattern of splenic B cells was heterogeneous, containing a substantial dim population (IBL-2(lo)), and a smaller, intensely stained fraction (IBL-2(hi)) within the positive subset. Unlike the B cells, the T cells were negative in every peripheral lymphoid tissue analysed. In addition, the ratios between the various IBL-2-reactive B cells (positive to negative and, within the positive population, the IBL-2(lo) to IBL-2(hi), respectively) in the spleen were quite similar to that of B cells in the bone marrow. Furthermore, the levels of L-selectin expressed by the various IBL-2-reactive subpopulations were found to be heterogeneous both in the bone marrow and in the spleen. The bone marrow cells could be resolved into double negative, L-selectin(±)/IBL-2(lo), L-selectin--IBL-2(lo), and L-selectin-/IBL-2(hi) populations, respectively. In the spleen, an additional fraction with L-selectin+/IBL-2- phenotype could be detected. In both tissues, the overwhelming majority of IBL-2(hi) cells were found at the MEL-14- compartment. We conclude that either these findings may reflect a heterogeneous development state within the peripheral B-cell pool, with a substantial fraction of splenic B cells being less differentiated than those in other peripheral lymphoid tissues, or alternatively, the differential reactivity of murine B cells with the IBL-2 monoclonal antibody is due to their tissue location.

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