TiProtec preserves endothelial function in a rat model

Gábor Veres, Péter Hegedus, Eniko Barnucz, Harald Schmidt, T. Radovits, Raphael Zöller, Matthias Karck, G. Szabó

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Coronary artery bypass surgery provides excellent patency rates; however, the early and/or late graft failure reduces the long-term benefit of myocardial revascularization. We investigated the effectiveness of generally used saline, Custodiol solutions and a new solution (TiProtec) at preserving endothelium after cold ischemia and warm reperfusion injury. Materials and methods: Aortic transplantations were performed in Lewis rats. Aortic arches were stored in saline, Custodiol, and TiProtec solutions for 2h then were transplanted into the abdominal aorta. Two, 24 hours and 1 week after transplantation, the implanted grafts were harvested. Endothelium-dependent and -independent vasorelaxations were investigated in organ bath. DNA strand breaks were assessed by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling-method, messenger RNA expressions by quantitative real-time polymerase chain reaction, and the expression of CD-31 and alpha smooth muscle actin by immunochemistry. Results: Severely impaired endothelial function and integrity of implanted aortic grafts were shown after 2 h in the saline, Custodiol group (maximal vasorelaxation to acetylcholine: control: 91±2%, saline: 26±5%, Custodiol: 24±5%, CD-31-positive area control: 96±2%, saline: 35±13% Custodiol: 54±5%, P

Original languageEnglish
JournalJournal of Surgical Research
DOIs
Publication statusAccepted/In press - May 8 2015

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Transplants
Vasodilation
Endothelium
Transplantation
Immunochemistry
Cold Ischemia
Myocardial Revascularization
DNA Breaks
DNA Nucleotidylexotransferase
Abdominal Aorta
Reperfusion Injury
Thoracic Aorta
Baths
Sodium Chloride
Coronary Artery Bypass
Acetylcholine
Smooth Muscle
Actins
Real-Time Polymerase Chain Reaction
Messenger RNA

Keywords

  • Bypass
  • Endothelium
  • Grafting
  • Ischemia
  • Reperfusion

ASJC Scopus subject areas

  • Surgery

Cite this

TiProtec preserves endothelial function in a rat model. / Veres, Gábor; Hegedus, Péter; Barnucz, Eniko; Schmidt, Harald; Radovits, T.; Zöller, Raphael; Karck, Matthias; Szabó, G.

In: Journal of Surgical Research, 08.05.2015.

Research output: Contribution to journalArticle

Veres, Gábor ; Hegedus, Péter ; Barnucz, Eniko ; Schmidt, Harald ; Radovits, T. ; Zöller, Raphael ; Karck, Matthias ; Szabó, G. / TiProtec preserves endothelial function in a rat model. In: Journal of Surgical Research. 2015.
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