TIP47 protects mitochondrial membrane integrity and inhibits oxidative-stress-induced cell death

E. Hocsak, B. Rácz, A. Szabó, L. Mester, E. Rapolti, E. Pozsgai, S. Javor, S. Bellyei, F. Gallyas, B. Sümegi, A. Szigeti

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We found that overexpression of tail interacting protein of 47kDa (TIP47), but not its truncated form (t-TIP47) protected NIH3T3 cells from hydrogen-peroxide-induced cell death, prevented the hydrogen-peroxide-induced mitochondrial depolarization determined by 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolylcarbocyanine iodide (JC1), while suppression of TIP47 in HeLa cells facilitated oxidative-stress-induced cell death. TIP47 was located to the cytoplasm of untreated cells, but some was associated to mitochondria in oxidative stress. Recombinant TIP47, but not t-TIP47 increased the mitochondrial membrane potential (Δψ), and partially prevented Ca2+ induced depolarization. It is assumed that TIP47 can bind to mitochondria in oxidative stress, and inhibit mitochondria mediated cell death by protecting mitochondrial membrane integrity.

Original languageEnglish
Pages (from-to)2953-2960
Number of pages8
JournalFEBS Letters
Volume584
Issue number13
DOIs
Publication statusPublished - Jul 2010

Fingerprint

Oxidative stress
Mitochondrial Membranes
Cell death
Oxidative Stress
Cell Death
Membranes
Mitochondria
Proteins
Depolarization
Hydrogen Peroxide
Mitochondrial Membrane Potential
Iodides
HeLa Cells
Cytoplasm

Keywords

  • Apoptosis
  • Hydrogen-peroxide
  • Mitochondrial permeability transition
  • Necrosis
  • Reactive oxygen species
  • Tail interacting protein of 47kDa

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

TIP47 protects mitochondrial membrane integrity and inhibits oxidative-stress-induced cell death. / Hocsak, E.; Rácz, B.; Szabó, A.; Mester, L.; Rapolti, E.; Pozsgai, E.; Javor, S.; Bellyei, S.; Gallyas, F.; Sümegi, B.; Szigeti, A.

In: FEBS Letters, Vol. 584, No. 13, 07.2010, p. 2953-2960.

Research output: Contribution to journalArticle

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AU - Rapolti, E.

AU - Pozsgai, E.

AU - Javor, S.

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AU - Szigeti, A.

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