TIP47 protects mitochondrial membrane integrity and inhibits oxidative-stress-induced cell death

E. Hocsak, B. Racz, A. Szabo, L. Mester, E. Rapolti, E. Pozsgai, S. Javor, S. Bellyei, F. Gallyas, B. Sumegi, A. Szigeti

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20 Citations (Scopus)

Abstract

We found that overexpression of tail interacting protein of 47kDa (TIP47), but not its truncated form (t-TIP47) protected NIH3T3 cells from hydrogen-peroxide-induced cell death, prevented the hydrogen-peroxide-induced mitochondrial depolarization determined by 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolylcarbocyanine iodide (JC1), while suppression of TIP47 in HeLa cells facilitated oxidative-stress-induced cell death. TIP47 was located to the cytoplasm of untreated cells, but some was associated to mitochondria in oxidative stress. Recombinant TIP47, but not t-TIP47 increased the mitochondrial membrane potential (Δψ), and partially prevented Ca2+ induced depolarization. It is assumed that TIP47 can bind to mitochondria in oxidative stress, and inhibit mitochondria mediated cell death by protecting mitochondrial membrane integrity.

Original languageEnglish
Pages (from-to)2953-2960
Number of pages8
JournalFEBS letters
Volume584
Issue number13
DOIs
Publication statusPublished - Jul 1 2010

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Keywords

  • Apoptosis
  • Hydrogen-peroxide
  • Mitochondrial permeability transition
  • Necrosis
  • Reactive oxygen species
  • Tail interacting protein of 47kDa

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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