Time-resolved photoelectric and absorption signals from oriented purple membranes immobilized in gel

András Dér, Pál Hargittai, József Simon

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80 Citations (Scopus)

Abstract

Kinetics of photoelectric and absorption response signals were measured on samples containing oriented purple membranes immobilized in polyacrylamide gel. The orientation and aggregation states of purple membranes remain constant independently of pH and ionic strength in such samples and the gel does not influence the protom pump. The 'gel method' described in this study enables direct investigation of proton pump of bacteriorhodopsin and a simultaneous measurement of absorption signals within a wide range of parameters of the solution surrounding purple membranes and offers possibilities for study of other types of membranes as well.

Original languageEnglish
Pages (from-to)295-300
Number of pages6
JournalJournal of biochemical and biophysical methods
Volume10
Issue number5-6
DOIs
Publication statusPublished - Mar 1985

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Keywords

  • gel
  • immobilization
  • orientation
  • photoelectric signal
  • purple membrane

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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