Time-lapse video microscopy and image analysis of adherence and growth patterns of Candida albicans strains

Gabor Nagy, Grant W. Hennig, Katalin Petrenyi, Laszlo Kovacs, I. Pócsi, V. Dombrádi, G. Bánfalvi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Digital image analysis of high time resolution video microscopy was used to investigate hyphal growth dynamics in different Candida albicans strains. The effects of the quorum sensing molecules tyrosol and farnesol, the deletion of the fungus specific protein phosphatase Z1 CaPPZ1), and the hypha-specific cyclin (HGC1) genes were analyzed by this method. Our system monitored cell growth in a CO2 incubator under near-physiological conditions and measured three major parameters under the following stringent conditions: (a) the time of yeast cell adherence, (b) the time of hyphal outgrowth, and (c) the rate of hyphal growth. This method showed that hyphal extension of wild-type SC5314 cells was accelerated by tyrosol and inhibited by farnesol. Hyphal growth rate was moderately lower in cappz1 and strongly reduced in hgc1 mutants. In addition, tyrosol treatment caused a firm adherence, while farnesol treatment and hgc1 mutation prevented the adherence of yeast cells to the surface of the culture flask. Transition from yeast-to-hyphal state was faster after tyrosol treatment, while it was reduced in farnesol-treated cells as well as in the cappz1 and hgc1 mutants. Our data confirm the notion that the attachment of yeast cells, the yeast-to-hyphal transition, and hyphal growth rate are closely related processes. Time-lapse video microscopy combined with image analysis offers a convenient and reliable method of testing chemicals, including potential drug candidates, and genetic manipulations on the dynamic morphological changes in C. albicans strains.

Original languageEnglish
Pages (from-to)5185-5194
Number of pages10
JournalApplied Microbiology and Biotechnology
Volume98
Issue number11
DOIs
Publication statusPublished - 2014

Fingerprint

Video Microscopy
Candida albicans
Farnesol
Yeasts
Growth
Incubators
Quorum Sensing
Cyclins
Hyphae
Phosphoprotein Phosphatases
Fungi
Mutation
4-hydroxyphenylethanol
Pharmaceutical Preparations
Genes

Keywords

  • Candida albicans
  • Genetic mutations
  • Hyphae outgrowth
  • Image analysis
  • Quorum-sensing molecules
  • Yeast cell adherence

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Medicine(all)

Cite this

Time-lapse video microscopy and image analysis of adherence and growth patterns of Candida albicans strains. / Nagy, Gabor; Hennig, Grant W.; Petrenyi, Katalin; Kovacs, Laszlo; Pócsi, I.; Dombrádi, V.; Bánfalvi, G.

In: Applied Microbiology and Biotechnology, Vol. 98, No. 11, 2014, p. 5185-5194.

Research output: Contribution to journalArticle

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