Time-lapse analysis of cell death in mammalian and fungal cells

Gabor Nagy, Gabor Pinter, Gabor Kohut, A. Ádám, Gyorgy Trencsenyi, L. Hornok, G. Bánfalvi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Time-lapse video microscopy was designed to follow the movement of single cells for an unlimited period of time under physiological conditions. The system is based on two inverted microscopes located in a CO2 incubator and equipped with charge-coupled device cameras connected to the computer. Frames were recorded every minute and the subsequent video sequence was converted to database form. The system was applied to describe the movements of normal HaCaT cells and Pb-treated cells causing the so-called apoptotic dance during cell death. The apoptotic movement was also followed in high-osmolarity glycerol-type mitogen-activated protein kinase (MAPK) null mutant of Fusarium proliferatum, a filamentous fungus, during osmotic stress. The shortest (20 min) and most vigorous death movements were observed in apoptotic fungal cells subjected to salt stress. The necrotic process at higher Pb concentration (50 μM) took 2-3 h, whereas the apoptotic process at lower Pb concentrations lasted from minutes to days.

Original languageEnglish
Pages (from-to)249-259
Number of pages11
JournalDNA and Cell Biology
Volume29
Issue number5
DOIs
Publication statusPublished - May 1 2010

Fingerprint

Cell Death
Incubators
Video Microscopy
Osmotic Pressure
Fusarium
Mitogen-Activated Protein Kinases
Osmolar Concentration
Glycerol
Cell Movement
Fungi
Salts
Databases
Equipment and Supplies

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Time-lapse analysis of cell death in mammalian and fungal cells. / Nagy, Gabor; Pinter, Gabor; Kohut, Gabor; Ádám, A.; Trencsenyi, Gyorgy; Hornok, L.; Bánfalvi, G.

In: DNA and Cell Biology, Vol. 29, No. 5, 01.05.2010, p. 249-259.

Research output: Contribution to journalArticle

Nagy, Gabor ; Pinter, Gabor ; Kohut, Gabor ; Ádám, A. ; Trencsenyi, Gyorgy ; Hornok, L. ; Bánfalvi, G. / Time-lapse analysis of cell death in mammalian and fungal cells. In: DNA and Cell Biology. 2010 ; Vol. 29, No. 5. pp. 249-259.
@article{1b9436f8ce9547818fe9c13d0d358e62,
title = "Time-lapse analysis of cell death in mammalian and fungal cells",
abstract = "Time-lapse video microscopy was designed to follow the movement of single cells for an unlimited period of time under physiological conditions. The system is based on two inverted microscopes located in a CO2 incubator and equipped with charge-coupled device cameras connected to the computer. Frames were recorded every minute and the subsequent video sequence was converted to database form. The system was applied to describe the movements of normal HaCaT cells and Pb-treated cells causing the so-called apoptotic dance during cell death. The apoptotic movement was also followed in high-osmolarity glycerol-type mitogen-activated protein kinase (MAPK) null mutant of Fusarium proliferatum, a filamentous fungus, during osmotic stress. The shortest (20 min) and most vigorous death movements were observed in apoptotic fungal cells subjected to salt stress. The necrotic process at higher Pb concentration (50 μM) took 2-3 h, whereas the apoptotic process at lower Pb concentrations lasted from minutes to days.",
author = "Gabor Nagy and Gabor Pinter and Gabor Kohut and A. {\'A}d{\'a}m and Gyorgy Trencsenyi and L. Hornok and G. B{\'a}nfalvi",
year = "2010",
month = "5",
day = "1",
doi = "10.1089/dna.2009.0980",
language = "English",
volume = "29",
pages = "249--259",
journal = "DNA and Cell Biology",
issn = "1044-5498",
publisher = "Mary Ann Liebert Inc.",
number = "5",

}

TY - JOUR

T1 - Time-lapse analysis of cell death in mammalian and fungal cells

AU - Nagy, Gabor

AU - Pinter, Gabor

AU - Kohut, Gabor

AU - Ádám, A.

AU - Trencsenyi, Gyorgy

AU - Hornok, L.

AU - Bánfalvi, G.

PY - 2010/5/1

Y1 - 2010/5/1

N2 - Time-lapse video microscopy was designed to follow the movement of single cells for an unlimited period of time under physiological conditions. The system is based on two inverted microscopes located in a CO2 incubator and equipped with charge-coupled device cameras connected to the computer. Frames were recorded every minute and the subsequent video sequence was converted to database form. The system was applied to describe the movements of normal HaCaT cells and Pb-treated cells causing the so-called apoptotic dance during cell death. The apoptotic movement was also followed in high-osmolarity glycerol-type mitogen-activated protein kinase (MAPK) null mutant of Fusarium proliferatum, a filamentous fungus, during osmotic stress. The shortest (20 min) and most vigorous death movements were observed in apoptotic fungal cells subjected to salt stress. The necrotic process at higher Pb concentration (50 μM) took 2-3 h, whereas the apoptotic process at lower Pb concentrations lasted from minutes to days.

AB - Time-lapse video microscopy was designed to follow the movement of single cells for an unlimited period of time under physiological conditions. The system is based on two inverted microscopes located in a CO2 incubator and equipped with charge-coupled device cameras connected to the computer. Frames were recorded every minute and the subsequent video sequence was converted to database form. The system was applied to describe the movements of normal HaCaT cells and Pb-treated cells causing the so-called apoptotic dance during cell death. The apoptotic movement was also followed in high-osmolarity glycerol-type mitogen-activated protein kinase (MAPK) null mutant of Fusarium proliferatum, a filamentous fungus, during osmotic stress. The shortest (20 min) and most vigorous death movements were observed in apoptotic fungal cells subjected to salt stress. The necrotic process at higher Pb concentration (50 μM) took 2-3 h, whereas the apoptotic process at lower Pb concentrations lasted from minutes to days.

UR - http://www.scopus.com/inward/record.url?scp=77952390949&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952390949&partnerID=8YFLogxK

U2 - 10.1089/dna.2009.0980

DO - 10.1089/dna.2009.0980

M3 - Article

C2 - 20218896

AN - SCOPUS:77952390949

VL - 29

SP - 249

EP - 259

JO - DNA and Cell Biology

JF - DNA and Cell Biology

SN - 1044-5498

IS - 5

ER -